Spalax fibroblast-derived anti-cancer agents

Abstract

A conditioned cell culture medium of cells derived from Spalax or naked mole rat ( Heterocephalus glaber ) and methods for preparing it are provided. Pharmaceutical compositions comprising the conditioned cell culture medium and its use in the treatment of cancer as well as methods for identifying anti-cancer agents are also provided.

1 . A method for treatment of cancer or inhibiting cancer metastasis in a human subject diagnosed with cancer, said method comprising administering to said subject an effective amount of a pharmaceutical composition comprising a conditioned cell culture medium of healthy cells derived from  Spalax  or naked mole rat ( Heterocephalus glaber ), and a pharmaceutically active carrier, excipient or diluent. 
     
     
         2 . The method according to  claim 1 , wherein said cells are derived from  Spalax ehrenbeigi  ( Nannospalax ). 
     
     
         3 . The method according to  claim 1 , wherein said cells are skin or lung fibroblasts. 
     
     
         4 . The method according to  claim 3 , wherein said fibroblasts are primary cells. 
     
     
         5 . The method according to claim I. wherein said cancer is selected from the group consisting of carcinoma, sarcoma, glioma, leukemia and lymphoma. 
     
     
         6 . The method according to  claim 5 , wherein said carcinoma is selected from the group consisting of breast cancer carcinoma, hepatocellular carcinoma and breast adenocarcinoma. 
     
     
         7 . The method according to  claim 1 , further including the concomitant administration of at least one chemotherapeutic agent. 
     
     
         8 . The method according to  claim 1 , wherein said composition further comprises at least one chemotherapeutic agent. 
     
     
         9 . A method for killing cancer cells, inhibiting proliferation of cancer cells or inhibiting cancer cell migration, comprising administering to a human subject in need thereof an effective amount of a pharmaceutical composition comprising a conditioned cell culture medium of healthy cells derived from  Spalax  or naked mole rat ( Heterocephalus glaber ), and a pharmaceutically active carrier, excipient or diluent. 
     
     
         10 . The method according to  claim 9 , wherein said cells are derived from  Spalax ehrenbergi  ( Nannospalax ). 
     
     
         11 . The method according to  claim 9 , wherein said cells are skin or lung fibroblasts. 
     
     
         12 . The method according to  claim 11 , wherein said fibroblasts are primary cells. 
     
     
         13 . The method according to  claim 9 , wherein said cancer cells are selected from the group consisting of carcinoma cells, sarcoma cells, glioma cells, leukemia cells and lymphoma cells. 
     
     
         14 . The method according to  claim 13 , wherein said carcinoma cells are selected from the group consisting of breast cancer carcinoma cells, hepatocellular carcinoma cells and breast adenocarcinoma cells. 
     
     
         15 . The method according to  claim 9 , further including the concomitant administration of at least one chemotherapeutic agent. 
     
     
         16 . The method according to  claim 9 , wherein said composition further comprises at east one chemotherapeutic agent.