Database and machine learning in response to parallel serial dual microfluidic chip

Abstract

A microfluidic chip device includes a reservoir for holding a biologic sample containing a predetermined biologic material. A first plurality of parallel pathways each testing one of one or more treatment-agents and analyzing a first efficacy for each of the one or more treatment agents applied to the predetermined biologic material within the biologic sample. A multiplexer provides a portion of the biologic sample into each of the first plurality of parallel pathways from the reservoir. A second plurality of parallel pathways each test a plurality of different dosage levels of the one or more treatment agents and analyzes a second efficacy of the plurality of different dosage levels with respect to the predetermined biologic material. The multiplexer further provides a second portion of the biologic sample into a selected at least one of the second plurality of parallel pathways responsive to a control input indicating the treatment agent providing a highest efficacy.

1 . A method for determining an efficacy of a treatment agent, comprising:
 holding a biologic sample containing a predetermined biologic material within a reservoir of a microfluidic chip device, pumping a portion of the biologic sample into each of a first plurality of parallel pathways of the microfluidic chip device from the reservoir using a first pump;   applying one or more treatment agents each within one of the first plurality of parallel pathways to the portion of the biologic sample within the parallel pathway;   analyzing a first efficacy of each of the applied one or more treatment agents to the portion of the biologic sample within each of the plurality of parallel pathways;   pumping a second portion of the biologic sample into a selected at least one second parallel pathway of a second plurality of parallel pathways from the reservoir using a second pump;   applying one of the one or more treatment agents at a plurality of different dosage levels within the selected at least one second parallel pathway to the second portion of the biologic sample within the selected at least one second parallel pathway; and   analyzing a second efficacy of each of the plurality of different dosage levels within each of the selected at least one second parallel pathway.   
     
     
         2 . The method of  claim 1 , wherein the step of pumping a second portion of the biologic sample further comprises pumping the second portion of the biologic sample into a plurality of parallel pathways, each of the plurality of parallel pathways applying a different dosage level of the plurality of different dosage levels. 
     
     
         3 . The method of  claim 1 , wherein the step of pumping a second portion of the biologic sample further comprises pumping the second portion of the biologic sample into a single parallel pathway, the single parallel pathway applying a different dosage level of the plurality of different dosage levels at different serial locations in the single parallel pathway. 
     
     
         4 . The method of  claim 1  further including the step of applying affinity labels to cells within the biologic sample. 
     
     
         5 . The method of  claim 1 , wherein the step of applying the one or more treatment agents further comprise passing the portion of the biologic sample through a serpentine microchannel to apply the treatment agent to the biologic sample. 
     
     
         6 . The method of  claim 1 , wherein the step of analyzing the first efficiency and the second efficiency further comprise displaying the biologic sample having the treatment agent applied thereto in a viewing reservoir. 
     
     
         7 . The method of  claim 1 , wherein the first plurality of parallel pathways of the microfluidic chip device are each associated with testing a first type of medical issue. 
     
     
         8 . A microfluidic chip device, comprising:
 a reservoir for holding a biologic sample containing a predetermined biologic material;   a first plurality of parallel pathways each for testing one of one or more treatment agents and for analyzing a first efficacy for each of the one or more treatment agents applied to the predetermined biologic material within the biologic sample;   a first pump for pumping a portion of the biologic sample into each of the first plurality of parallel pathways from the reservoir;   a second plurality of parallel pathways each for testing a plurality of different dosage levels of the one or more treatment agents and for analyzing a second efficacy of the plurality of different dosage levels with respect to the predetermined biologic material; and   a plurality of second pumps each associated with one of the second plurality of parallel pathways for pumping a second portion of the biologic sample into a selected at least one of the second plurality of parallel pathways responsive to a control input indicating the treatment agent providing a highest efficacy.   
     
     
         9 . The method of  claim 8 , wherein the plurality of second pumps the second portion of the biologic sample into a plurality of parallel pathways, each of a plurality of parallel pathways of the second plurality of parallel pathways to apply a different dosage level of the plurality of different dosage levels. 
     
     
         10 . The microfluidic chip device of  claim 8 , wherein the plurality of second pumps pump the second portion of the biologic sample into a single parallel pathway of the plurality of parallel pathways, the single parallel pathway applying a different dosage level of the plurality of different dosage levels at different serial locations in the single parallel pathway. 
     
     
         11 . The microfluidic chip device of  claim 8  further including a medium contained within the reservoir containing various affinity labels for applying affinity labels to cells within the biologic sample. 
     
     
         12 . The microfluidic chip device of  claim 8 , wherein the first plurality of pathways each further includes a first serpentine microchannel for applying the treatment agent to the biologic sample. 
     
     
         13 . The microfluidic chip device of  claim 8 , wherein the second plurality of pathways each further includes a second serpentine microchannel for applying the treatment agent to the biologic sample at the predetermined dosage. 
     
     
         14 . The microfluidic chip device of  claim 8 , wherein the first plurality of pathways and the second plurality of pathways further include a viewing reservoir for analyzing the first efficiency and the second efficiency. 
     
     
         15 . The microfluidic chip device of  claim 8 , wherein the first plurality of parallel pathways of the microfluidic chip device are each associated with testing a first type of medical issue. 
     
     
         16 . A microfluidic chip device, comprising:
 a reservoir for holding a biologic sample containing a predetermined biologic material;   a first plurality of parallel pathways each for testing one of one or more treatment agents and for analyzing a first efficacy for each of the one or more treatment agents applied to the predetermined biologic material within the biologic sample;   a multiplexer for providing a portion of the biologic sample into each of the first plurality of parallel pathways from the reservoir;   a second plurality of parallel pathways each for testing a plurality of different dosage levels of the one or more treatment agents and for analyzing a second efficacy of the plurality of different dosage levels with respect to the predetermined biologic material; and   wherein the multiplexer further provides a second portion of the biologic sample into a selected at least one of the second plurality of parallel pathways responsive to a control input indicating the treatment agent providing a highest efficacy.   
     
     
         17 . The microfluidic chip device of  claim 16 , wherein the multiplexer provides the second portion of the biologic sample into a plurality of parallel pathways, each of a plurality of parallel pathways of the second plurality of parallel pathways to apply a different dosage level of the plurality of different dosage levels. 
     
     
         18 . The microfluidic chip device of  claim 16 , wherein the multiplexer provides the second portion of the biologic sample into a single parallel pathway of the plurality of parallel pathways, the single parallel pathway applying a different dosage level of the plurality of different dosage levels at different serial locations in the single parallel pathway. 
     
     
         19 . The microfluidic chip device of  claim 8 , wherein the first plurality of pathways each further includes a first serpentine microchannel for applying the treatment agent to the biologic sample. 
     
     
         20 . The microfluidic chip device of  claim 8 , wherein the first plurality of parallel pathways of the microfluidic chip device are each associated with testing a first type of medical issue.