Affinity-oligonucleotide conjugates and uses thereof

Abstract

Provided herein are methods and compositions for single cell characterization using affinity-oligonucleotide conjugates.

1 - 124 . (canceled) 
     
     
         125 . A kit for making conjugates, comprising:
 (a) a first container comprising a first oligonucleotide comprising a first antigen identification (AID) sequence and a first affinity molecular barcode (AMB) sequence, wherein the first AID sequence and the first AMB sequence are known sequences;   (b) a second container comprising a second oligonucleotide comprising a second antigen identification (AID) sequence and a second affinity molecular barcode (AMB) sequence, wherein the second AID sequence is a known sequence and is different from the first AID sequence, and wherein the second AMB sequence is a known sequence and is different from the first AMB sequence; and   (c) one or more additional containers comprising reagents capable of conjugating the first oligonucleotide to a first affinity molecule and/or comprising reagents capable of conjugating the second oligonucleotide to a second affinity molecule;   wherein the first affinity molecule is configured to bind to a first extracellular protein of a cell, and the second affinity molecule is configured to bind to a second extracellular protein of a cell, wherein the first extracellular protein is different from the second extracellular protein; and   (d) a set of instructions describing how to conjugate the first oligonucleotide to the first affinity molecule and the second oligonucleotide to the second affinity molecule.   
     
     
         126 . The kit of  claim 125 , further comprising:
 (e) a further container comprising a plurality of vessel barcoded polynucleotides, wherein each vessel barcoded polynucleotide of the plurality of vessel barcoded polynucleotides comprises a unique vessel barcoded sequence; and   (f) reagents and a set of instructions to attach a vessel barcoded polynucleotide of the plurality of vessel barcoded polynucleotides to the first or second oligonucleotide when conjugated to the first or second affinity molecule.