Nematode resistance alleles in soybean

Abstract

Methods for conveying soy cyst nematode (SCN) resistance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing SCN resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with SCN resistance. Also provided are single nucleotide polymorphisms (SNPs) associated with resistance to SCN. Soybean plants and seeds produced by any of the disclosed methods are provided.

That which is claimed: 
     
         1 . A method of identifying or selecting an SCN tolerant soybean plant or part thereof, comprising the steps of
 a) Isolating a nucleic acid from a first soybean plant;   b) Detecting in the nucleic acid of a) a molecular marker associated with increased SCN resistance wherein said molecular marker corresponds to any one of Chromosome 18 a G at position 1638718; Chromosome 18 a G at position 1690438; Chromosome 18 a C at position 1712035; Chromosome 18 a G at position 1712922; Chromosome 18 a C at position 1735950; Chromosome 18 a A at position 1736100; Chromosome 18 a G at position 1736136; Chromosome 19 a A at position 37734309; or Chromosome 19 a G at position 37877119.   c) thereby identifying or selecting a SCN tolerant soybean plant or part thereof.   
     
     
         2 . A method of producing a SCN resistant soybean plant, the method comprising the steps of:
 a) Crossing a first soybean plant with a second soybean plant wherein said first plant comprises in its genome a SCN resistance loci further wherein the SCN resistance loci comprises any one of any one of Chromosome 18 a G at position 1638718; Chromosome 18 a G at position 1690438; Chromosome 18 a C at position 1712035; Chromosome 18 a G at position 1712922; Chromosome 18 a C at position 1735950; Chromosome 18 a A at position 1736100; Chromosome 18 a G at position 1736136; Chromosome 19 a A at position 37734309; or Chromosome 19 a G at position 37877119 and wherein said second plant does not comprise said SCN resistance loci;   b) producing a progeny seed from the cross of a);   c) growing a progeny plant from the seed of b) wherein said progeny plant comprises in its genome the SCN resistance loci described in a) thereby producing a SCN resistant plant.   
     
     
         3 . The method of  claim 3 , wherein the first soybean plant is CE1210290. 
     
     
         4 . A plant produced by the method of  claim 3 . 
     
     
         5 . A method of identifying or selecting an SCN tolerant soybean plant or part thereof, comprising the steps of
 a) Isolating a nucleic acid from a first soybean plant;   b) Detecting in the nucleic acid of a) a molecular marker within a chromosomal interval associated with increased SCN resistance wherein said chromosomal interval is defined by and including Chromosome 19 position 37734309 to position 37877119.   c) thereby identifying or selecting a SCN tolerant soybean plant or part thereof.   
     
     
         6 . A method of producing a SCN resistant soybean plant, the method comprising the steps of:
 a) Crossing a first soybean plant with a second soybean plant wherein said first plant comprises in its genome a chromosomal interval wherein the chromosomal interval is defined by and including Chromosome 19 position 37734309 to position 37877119 and wherein said second plant does not comprise said chromosomal interval;   b) producing a progeny seed from the cross of a);   c) growing a progeny plant from the seed of b) wherein said progeny plant comprises in its genome the chromosomal interval of a) thereby producing a SCN resistant plant.   
     
     
         7 . The method of  claim 6 , wherein the first soybean plant is CE1210290 or a progeny thereof. 
     
     
         8 . The method of  claim 7 , wherein the progeny plant produced in c) shows increased resistance to SCN race 2. 
     
     
         9 . A plant created by the method of  claim 7 . 
     
     
         10 . A elite soybean plant having introgressed into its genome a chromosomal interval from CE1210290 or a progeny thereof wherein the chromosomal interval comprises at least one molecular marker associated with SCN resistance wherein the molecular marker corresponds to soybean chromosome 19 position 37734309 having a A or soybean chromosome position 37877119 having a G.