Bioreactor System

Abstract

A three dimensional cell culture and bioreactor system is provided. The system comprises one or more cell culture chamber. Each cell culture chamber comprises an inlet port and an outlet port in fluid communication with the cell culture chamber. The cell culture chambers may be segregated or in fluid communication with one another. The systems may be used to conduct drug efficacy test, isolate certain cell types from a complex tissue sample of multiple cell types, allow for the ex vivo culturing of patient tissue samples to help guide the course of treatment, and conduct co-culture experiments.

1 - 29 . (canceled) 
     
     
         30 . A three dimensional cell culture and bioreactor system comprising:
 a cell culture chamber comprising an inlet port and an outlet port that are in communication with an interior volume of the cell culture chamber, wherein the interior volume of the cell culture chamber is configured to house a target cell population; and   a cell-scaffold construct housed in the cell culture chamber, wherein the scaffold portion of the cell-scaffold construct is made from a combination of a porous stiff culture material having a modulus greater than 1 GPa and a soft material having a modulus of less than 500 MPa, wherein the porous stiff culture material has a pore size ranging from 400 micrometers to 2 millimeters.