US2022002740A2PendingUtilityA2

Mutations in mads-box genes and uses thereof

29
Assignee: LIPPMAN ZACHARYPriority: May 17, 2017Filed: May 17, 2018Published: Jan 6, 2022
Est. expiryMay 17, 2037(~10.8 yrs left)· nominal 20-yr term from priority
Inventors:Zachary LippmanSebastian SoykZachary Lemmon
C12N 2310/20A01H 6/825C07K 14/415A01H 5/10C12N 9/22C12N 15/8213A01H 5/08
29
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Claims

Abstract

Aspects of the disclosure relate to plants, such as Solanaceae plants containing one or more of a mutant Solyc04g005320 gene (or a homolog thereof), a mutant Solycl2g038510 gene (or a homolog thereof), and a mutant Solyc03gl14840 gene (or a homolog thereof), as well as methods of producing such plants. In some aspects, such plants have one or more improved traits, such as modified inflorescence architecture, modified flower number, modified fruit number, higher yield, higher quality products, and higher fruit productivity.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . A genetically-altered Solanaceae plant comprising a mutant Solyc04g005320 gene or a homolog thereof. 
     
     
         2 . The genetically-altered Solanaceae plant of  claim 1 , wherein the mutant Solyc04g005320 gene or homolog thereof is a null allele or a hypomorphic allele. 
     
     
         3 . The genetically-altered Solanaceae plant of  claim 1 , wherein the genetically-altered Solanaceae plant is heterozygous or homozygous for the mutant Solyc04g005320 gene or homolog thereof. 
     
     
         4 . The genetically-altered Solanaceae plant of  claim 1 , wherein the genetically-altered Solanaceae plant further comprises a mutant Solyc12g038510 gene or a homolog thereof, a mutant Solyc03g114840 gene or a homolog thereof, or both a mutant Solyc12g038510 gene or a homolog thereof and a mutant Solyc03g114840 gene or a homolog thereof. 
     
     
         5 . The genetically-altered Solanaceae plant of  claim 1 , wherein the plant further comprises a mutant Solyc12g038510 gene or homolog thereof and the mutant Solyc12g038510 gene or homolog thereof is a null allele or a hypomorphic allele. 
     
     
         6 . The genetically-altered Solanaceae plant of  claim 5 , wherein the genetically-altered Solanaceae plant is heterozygous or homozygous for the mutant Solyc12g038510 gene or homolog thereof. 
     
     
         7 . The genetically-altered Solanaceae plant of  claim 1 , wherein the plant further comprises a mutant Solyc03g114840 gene or a homolog thereof and the mutant Solyc03g114840 gene or homolog thereof is a null allele or a hypomorphic allele. 
     
     
         8 . The genetically-altered Solanaceae plant of  claim 7 , wherein the genetically-altered Solanaceae plant is heterozygous or homozygous for the mutant Solyc03g114840 gene or homolog thereof. 
     
     
         9 . The genetically-altered Solanaceae plant of  claim 1 , wherein the plant further comprises both a mutant Solyc12g038510 gene or a homolog thereof and a mutant Solyc03g114840 gene or a homolog thereof, each of which are independently a null allele or a hypomorphic allele. 
     
     
         10 . The genetically-altered Solanaceae plant of  claim 9 , wherein the genetically-altered Solanaceae plant is heterozygous or homozygous for the mutant Solyc12g038510 gene or homolog thereof and is heterozygous or homozygous for the mutant Solyc03g114840 gene or homolog thereof. 
     
     
         11 . The genetically-altered Solanaceae plant of  claim 1 , wherein the genetically-altered Solanaceae plant further comprises a mutant Solyc12g038510 gene or homolog thereof, and a mutant Solyc03g114840 gene or homolog thereof, and wherein each is a hypomorphic allele. 
     
     
         12 . A genetically-altered Solanaceae plant, comprising a mutant Solyc12g038510 gene or a homolog thereof and a mutant Solyc03g114840 gene or a homolog thereof, wherein the genetically-altered Solanaceae plant is homozygous for the mutant Solyc12g038510 gene or homolog thereof and heterozygous for the mutant Solyc03g114840 gene or homolog thereof. 
     
     
         13 . The genetically-altered Solanaceae plant of  claim 12 , wherein the mutant Solyc12g038510 gene or homolog thereof is a null allele or a hypomorphic allele and the mutant Solyc03g114840 gene or homolog thereof is a null allele or a hypomorphic allele. 
     
     
         14 . The genetically-altered Solanaceae plant of  claim 1 , wherein the genetically-altered Solanaceae plant is a tomato ( Solanum lycopersicum ) plant. 
     
     
         15 . The genetically-altered Solanaceae plant of  claim 4 , wherein the mutant Solyc04g005320 gene or homolog thereof, the mutant Solyc12g038510 gene or homolog thereof, and/or the mutant Solyc03g114840 gene or homolog thereof is introduced by technical means. 
     
     
         16 . The genetically-altered Solanaceae plant of  claim 4 , wherein the mutant Solyc04g005320 gene or homolog thereof, the mutant Solyc12g038510 gene or homolog thereof, and/or the mutant Solyc03g114840 gene or homolog thereof is introduced by chemical or physical means. 
     
     
         17 . The genetically-altered Solanaceae plant of  claim 16 , wherein the mutant Solyc04g005320 gene or homolog thereof, the mutant Solyc12g038510 gene or homolog thereof, and/or the mutant Solyc03g114840 gene or homolog thereof is introduced using CRISPR/Cas9, chemical mutagenesis, radiation,  Agrobacterium -mediated recombination, viral-vector mediated recombination, or transposon mutagenesis. 
     
     
         18 . (canceled) 
     
     
         19 . A crop harvested from genetically-altered Solanaceae plants as defined in  claim 1 . 
     
     
         20 . A seed for producing a genetically-altered Solanaceae plant of  claim 1 . 
     
     
         21 . A method for producing a genetically-altered Solanaceae plant, the method comprising introducing a mutation into a Solyc04g005320 gene or a homolog thereof in a Solanaceae plant, thereby producing a genetically-altered Solanaceae plant containing a mutant Solyc04g005320 gene or homolog thereof. 
     
     
         22 . The method of  claim 21 , wherein the mutation is introduced using CRISPR/Cas9. 
     
     
         23 . The method of  claim 21 , wherein the mutation produces a null allele or a hypomorphic allele of the Solyc04g005320 gene or homolog thereof. 
     
     
         24 . The method of  claim 21 , wherein the method further comprises introducing into the Solanaceae plant a mutation into a Solyc12g038510 gene or a homolog thereof, introducing a mutation into a Solyc03g114840 gene or a homolog thereof, or introducing the mutation into the Solyc12g038510 gene or homolog thereof and introducing the mutation into the Solyc03g114840 gene or homolog thereof. 
     
     
         25 . The method of  claim 24 , wherein the mutation(s) is/are introduced using CRISPR/Cas9. 
     
     
         26 . The method of  claim 21 , wherein the genetically-altered Solanaceae plant containing the mutant Solyc04g005320 gene or homolog thereof is crossed to another genetically-altered Solanaceae plant comprising a mutant Solyc12g038510 gene or homolog thereof, a mutant Solyc03g114840 gene or homolog thereof, or both the mutant Solyc12g038510 gene or homolog thereof and the mutant Solyc03g114840 gene or homolog thereof, thereby producing a genetically-altered Solanaceae plant containing the mutant Solyc04g005320 gene or homolog thereof and the mutant Solyc12g038510 gene or homolog thereof, the mutant Solyc03g114840 gene or homolog thereof, or both the mutant Solyc12g038510 gene or homolog thereof and the mutant Solyc03g114840 gene or homolog thereof. 
     
     
         27 . The method of  claim 21 , wherein the genetically-altered Solanaceae plant is a tomato ( Solanum lycopersicum ) plant. 
     
     
         28 . A genetically-altered Solanaceae plant produced or obtainable by the method of  claim 21 . 
     
     
         29 . The genetically-altered Solanaceae plant of  claim 1 , wherein the mutant Solyc04g005320 gene or homolog thereof is a hypermorphic allele. 
     
     
         30 . The genetically-altered Solanaceae plant of  claim 29 , wherein the genetically-altered Solanaceae plant is heterozygous or homozygous for the mutant Solyc04g005320 gene or homolog thereof. 
     
     
         31 . (canceled)

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