Compositions and Methods of Use of Novel Strains of Lactobacillus Fermentum

Abstract

Provided herein are novel Lactobacillus fermentum strains, NRRL B-67059 or NRRL B-67060, compositions containing the NRRL B-67059 or NRRL B-67060 strains, and fermented dairy products and bioactive compounds prepared using the NRRL B-67059 or NRRL B-67060 strains. Also provided are methods of modulating the immune system of a subject.

1 - 44 . (canceled) 
     
     
         45 . A composition comprising one or more  Lactobacillus fermentum  strains that share at least 80% to 100% genome sequence identity with  Lactobacillus fermentum  strain NRRL B-67059 and/or  Lactobacillus fermentum  strain NRRL B-67060. 
     
     
         46 . The composition of  claim 45 , further comprising a bioactive agent, an additive, an excipient, an emulsifier, a therapeutic agent, a pharmaceutically acceptable carrier, and/or a preservative. 
     
     
         47 . The composition of  claim 45 , formulated as a pharmaceutical composition or edible product. 
     
     
         48 . The composition of  claim 47 , wherein the pharmaceutical composition is a solid dosage form, a semisolid dosage form, or a liquid dosage form. 
     
     
         49 . The composition of  claim 48 , wherein the solid dosage form is a tablet, a lozenge, or a capsule. 
     
     
         50 . The composition of  claim 49 , wherein the tablet or the capsule comprises microtablets, minitablets, pellets, or granulates comprising lyophilized powder of the strain. 
     
     
         51 . The composition of  claim 48 , wherein the semisolid dosage form is a gel, a cream, an ointment, or a chewable lozenge. 
     
     
         52 . The composition of  claim 48 , wherein the liquid is an emulsion or a solution. 
     
     
         53 . The composition of  claim 45 , formulated for oral, rectal, or parenteral administration. 
     
     
         54 . A method of treating a disease or disorder selected from the group consisting of: a dietary disease or disorder, an inflammatory disease or disorder, and an autoimmune disease or disorder in a subject in need thereof, the method comprising administering to the subject an effective amount of the composition of  claim 45 . 
     
     
         55 . A method of modulating the immune system in a subject in need thereof, the method comprising administering to the subject an effective amount of the composition of  claim 45 . 
     
     
         56 . A method of improving health in a subject in need thereof, the method comprising administering to the subject an effective amount of the composition of  claim 45 . 
     
     
         57 . The method of  claim 56 , wherein the improving the health of the subject is achieved by modulating the immune system of the subject. 
     
     
         58 . A method of preparing a food product, a food supplement, or a pharmaceutical composition comprising a dairy bioactive fraction of one or more  Lactobacillus fermentum  strains that shares at least 80% to 100% genome sequence identity with  Lactobacillus fermentum  strain NRRL B-67059 and/or  Lactobacillus fermentum  strain NRRL B-67060, wherein the bioactive fraction is obtained by a bioguided procedure for preparing the food product, the food supplement, or the pharmaceutical composition. 
     
     
         59 . A process for preparing an edible product, the method comprising fermenting a protein containing starting material with one or more  Lactobacillus fermentum  strains that shares at least 80% to 100% genome sequence identity with  Lactobacillus fermentum  strain NRRL B-67059 and/or  Lactobacillus fermentum  strain NRRL B-67060. 
     
     
         60 . A method of modulating the immune system in a subject in need thereof, the method comprising administering to the subject an effective amount of an edible product prepared by the process of  claim 59 . 
     
     
         61 . A method of treating, preventing, and/or ameliorating inflammation in a subject in need thereof, the method comprising administering to the subject an effective amount of an edible product prepared by the process of  claim 59 . 
     
     
         62 . A method of improving the immune system in a subject in need thereof, the method comprising administering to the subject an effective amount of a composition comprising one or more  Lactobacillus fermentum  strains that shares at least 80% to 100% genome sequence identity with  Lactobacillus fermentum  strain NRRL B-67059 and/or  Lactobacillus fermentum  strain NRRL B-67060. 
     
     
         63 . The method of  claim 62 , wherein the composition is formulated as a pharmaceutical composition or edible product. 
     
     
         64 . A method of enhancing secretion of cytokines in a subject in need thereof, the method comprising administering to the subject an effective amount of the composition of  claim 45 .