US2021395801A1PendingUtilityA1

Oligonucleotide primers for quantifying and/or detecting human male dna and kits containing the same

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Assignee: QIAGEN GMBHPriority: Sep 16, 2016Filed: Jul 29, 2021Published: Dec 23, 2021
Est. expirySep 16, 2036(~10.2 yrs left)· nominal 20-yr term from priority
Inventors:Miroslav VranesRalf PeistMario SchererStefan Otto CorneliusMargaretha König
C12Q 1/6851C12Q 2525/151C12Q 1/6827C12Q 2600/16C12Q 1/686C12Q 1/6879C12Q 2600/166C12Q 1/6806C12Q 1/6876
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Claims

Abstract

Oligonucleotide primers and probes for quantifying and/or detecting human male DNA in a forensic sample. The oligonucleotides are useful for amplifying a multicopy locus within the human Y-chromosome (MCL-Y) that shares at least 80% sequence identity to a sequence according to SEQ ID NO. 3 over a stretch of at least 60 base pairs. The oligonucleotides hybridize under stringent conditions to a nucleic acid having at least one sequence selected from the group consisting of SEQ ID NO. 3 to SEQ ID NO. 11 and/or SEQ ID NO. 17 to SEQ ID NO. 25. Kits including the oligonucleotide primers, probes and/or primer pairs and reagents for performing an amplification reaction on DNA recovered from a forensic sample are also disclosed.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . An oligonucleotide primer or probe that hybridizes under stringent conditions to a nucleic acid having at least one sequence selected from the group consisting of:
 SEQ ID NO. 3;   SEQ ID NO. 4;   SEQ ID NO. 5;   SEQ ID NO. 6;   SEQ ID NO. 7;   SEQ ID NO. 8;   SEQ ID NO. 9;   SEQ ID NO. 10;   SEQ ID NO. 11;   SEQ ID NO. 17;   SEQ ID NO. 18;   SEQ ID NO. 19;   SEQ ID NO. 20;   SEQ ID NO. 21;   SEQ ID NO. 22;   SEQ ID NO. 23;   SEQ ID NO. 24; and   SEQ ID NO. 25.   
     
     
         2 . The oligonucleotide primer or probe according to  claim 1 , wherein the oligonucleotide primer or probe is selected from the group consisting of:
 SEQ ID NO. 1;   SEQ ID NO. 2;   SEQ ID NO. 12;   SEQ ID NO. 13;   SEQ ID NO. 14;   SEQ ID NO. 15; and   SEQ ID NO. 16.   
     
     
         3 . The oligonucleotide primer or probe according to  claim 1 , wherein the oligonucleotide primer or probe is selected from the group consisting of:
 the reverse complement of SEQ ID NO. 1;   the reverse complement of SEQ ID NO. 2;   the reverse complement of SEQ ID NO. 12;   the reverse complement of SEQ ID NO. 13;   the reverse complement of SEQ ID NO. 14;   the reverse complement of SEQ ID NO. 15; and   the reverse complement of SEQ ID NO. 16.   
     
     
         4 . The oligonucleotide primer or probe according to  claim 1 , wherein the oligonucleotide primer or probe is selected from the group consisting of:
 a primer that shares at least 90% sequence identity with SEQ ID NO. 1;   a primer that shares at least 90% sequence identity with SEQ ID NO. 2;   a probe that shares at least 90% sequence identity with SEQ ID NO. 12;   a primer that shares at least 90% sequence identity with SEQ ID NO. 13;   a primer that shares at least 90% sequence identity with SEQ ID NO. 14;   a primer that shares at least 90% sequence identity with SEQ ID NO. 15; and   a probe that shares at least 90% sequence identity with SEQ ID NO. 16.   
     
     
         5 . The oligonucleotide primer according to  claim 1 , wherein the oligonucleotide primer or probe is selected from the group consisting of:
 the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 1;   the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 2;   the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 12;   the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 13;   the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 14;   the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 15; and   the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 16.   
     
     
         6 . An oligonucleotide primer pair selected from the group consisting of:
 SEQ ID NO. 1 and SEQ ID NO. 2;   SEQ ID NO. 2 and SEQ ID NO. 13;   SEQ ID NO. 2 and SEQ ID NO. 14;   SEQ ID NO. 2 and SEQ ID NO. 15;   the reverse complement of SEQ ID NO. 1 and the reverse complement of SEQ ID NO. 2;   the reverse complement of SEQ ID NO. 2 and the reverse complement of SEQ ID NO. 13;   the reverse complement of SEQ ID NO. 2 and the reverse complement of SEQ ID NO. 14;   the reverse complement of SEQ ID NO. 2 and the reverse complement of SEQ ID NO. 15;   a primer that shares at least 90% sequence identity with SEQ ID NO. 1 and a primer that shares at least 90% sequence identity with SEQ ID NO. 2;   a primer that shares at least 90% sequence identity with SEQ ID NO. 2 and a primer that shares at least 90% sequence identity with SEQ ID NO. 13;   a primer that shares at least 90% sequence identity with SEQ ID NO. 2 and a primer that shares at least 90% sequence identity with SEQ ID NO. 14;   a primer that shares at least 90% sequence identity with SEQ ID NO. 2 and a primer that shares at least 90% sequence identity with SEQ ID NO. 15;   the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 1 and the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 2;   the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 2 and the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 13;   the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 2 and the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 14; and   the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 2 and the reverse complement of a primer that shares at least 90% sequence identity with SEQ ID NO. 15.   
     
     
         7 . The oligonucleotide primer pair according to  claim 6 , wherein the primer pair is a primer that shares at least 90% sequence identity with SEQ ID NO. 1 and a primer that shares at least 90% sequence identity with SEQ ID NO. 2, or the reverse complements thereof. 
     
     
         8 . The oligonucleotide primer pair according to  claim 6 , wherein the primer pair is a primer that shares at least 90% sequence identity with SEQ ID NO. 2 and a primer that shares at least 90% sequence identity with SEQ ID NO. 13, or the reverse complements thereof. 
     
     
         9 . The oligonucleotide primer pair according to  claim 6 , wherein the primer pair is a primer that shares at least 90% sequence identity with SEQ ID NO. 2 and a primer that shares at least 90% sequence identity with SEQ ID NO. 14, or the reverse complements thereof. 
     
     
         10 . The oligonucleotide primer pair according to  claim 6 , wherein the primer pair is a primer that shares at least 90% sequence identity with SEQ ID NO. 2 and a primer that shares at least 90% sequence identity with SEQ ID NO. 15, or the reverse complements thereof. 
     
     
         11 . A kit comprising an oligonucleotide primer or probe according to  claim 1  and reagents for performing an amplification reaction on DNA recovered from a forensic sample. 
     
     
         12 . A kit comprising an oligonucleotide primer or probe according to  claim 2  and reagents for performing an amplification reaction on DNA recovered from a forensic sample. 
     
     
         13 . A kit comprising an oligonucleotide primer or probe according to  claim 3  and reagents for performing an amplification reaction on DNA recovered from a forensic sample. 
     
     
         14 . A kit comprising an oligonucleotide primer or probe according to  claim 4  and reagents for performing an amplification reaction on DNA recovered from a forensic sample. 
     
     
         15 . A kit comprising an oligonucleotide primer or probe according to  claim 5  and reagents for performing an amplification reaction on DNA recovered from a forensic sample. 
     
     
         16 . A kit comprising an oligonucleotide primer pair according to  claim 6  and reagents for performing an amplification reaction on DNA recovered from a forensic sample. 
     
     
         17 . A kit comprising an oligonucleotide primer pair according to  claim 7  and reagents for performing an amplification reaction on DNA recovered from a forensic sample. 
     
     
         18 . A kit comprising an oligonucleotide primer pair according to  claim 8  and reagents for performing an amplification reaction on DNA recovered from a forensic sample. 
     
     
         19 . A kit comprising an oligonucleotide primer pair according to  claim 9  and reagents for performing an amplification reaction on DNA recovered from a forensic sample. 
     
     
         20 . A kit comprising an oligonucleotide primer pair according to  claim 10  and reagents for performing an amplification reaction on DNA recovered from a forensic sample.

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