Transgenic plant having herbicide resistance

Abstract

The invention is intended to identify glutathione-S-transferase that exhibits the activities to metabolize and detoxify an isoxazoline derivative, such as pyroxasulfone. The invention provides a method for cultivating a transgenic plant into Which a nucleic acid encoding a protein (a or b) below has been introduced in the presence of isoxazoline derivatives:(a) a protein comprising the amino acid sequence as shown in SEQ ID NO: 2; or(b) a protein comprising an amino acid sequence having 80% or higher identity to the amino acid sequence as shown in SEQ ID NO: 2 and having the activity of glutathione-S-transferase.

1 . A transgenic plant into which a nucleic acid encoding a protein (a) or (b) below has been introduced:
 (a) a protein comprising the amino acid sequence as shown in SEQ ID NO: 2; or   (b) a protein comprising an amino acid sequence having 50% or higher identity to the amino acid sequence as shown in SEQ ID NO: 2 and having the activity of glutathione-S-transferase; wherein the transgenic plant has 10 times or higher isoxazoline derivative resistance than that of wild-type plants.   
     
     
         2 . The transgenic plant according to  claim 1 . which has resistance to an isoxazoline derivative and/or resistance to environmental stress. 
     
     
         3 . The transgenic plant according to  claim 1 , which results from introduction of the nucleic acid into a first plant having sensitivity to an isoxazoline derivative relative to the transgenic plant. 
     
     
         4 . The transgenic plant according to  claim 2 , wherein the isoxazoline derivative is pyroxasulfone and/or fenoxasulfone. 
     
     
         5 . The transgenic plant according to  claim 2 , wherein the environmental stress is high temperature stress. 
     
     
         6 . The transgenic plant according to  claim 3 , wherein the first plant having sensitivity to an isoxazoline derivative is a Gramineae. 
     
     
         7 . The transgenic plant according to  claim 6 , wherein the first plant of the Gramineae is rice. 
     
     
         8 . The transgenic plant according to  claim 3 , wherein the first plant having sensitivity to an isoxazoline derivative is a Brassicaceae, a Leguminosae, a Umbelliferae, a Amaranthaceae, a Labiatae, a Chenopodiaceae, a Rosaceae, a Compositae, a Solanaceae, or a Malvaceae. 
     
     
         9 . The transgenic plant according to  claim 8 , wherein the plant is Brassica napus or  Arabidopsis thaliana.