Medical devices coated with polydopamine and antibodies

Abstract

The present disclosure provides for a medical device or a substrate coated with polydopamine which is further linked to ligands such as antibodies and/or antibody fragments. The polydopamine coating and the ligands may be linked through a linker such as an organic polymer.

What is claimed is: 
     
         1 . A medical device having a coating, wherein the coating comprises (i) polydopamine, (ii) a polyether derivative, and (iii) antibodies and/or antibody fragments, wherein the polydopamine is coated on the medical device and is covalently linked to the polyether derivative, wherein the polyether derivative is covalently linked to the antibodies and/or antibody fragments, wherein the antibodies and/or antibody fragments specifically bind to a cell surface antigen of endothelial progenitor cells or endothelial cells, and wherein the cell surface antigen is CD34. 
     
     
         2 . The medical device of  claim 1 , wherein the polyether derivative is polyethylene glycol (PEG), a polyethylene glycol (PEG) derivative, polypropylene glycol (PPG), a polypropylene glycol (PPG) derivative, or combinations thereof. 
     
     
         3 . The medical device of  claim 1 , wherein the polyether derivative has an average molecular weight ranging from 200 Daltons to 1,000 Daltons. 
     
     
         4 . The medical device of  claim 3 , wherein the polyether derivative has an average molecular weight ranging from 200 Daltons to 350 Daltons. 
     
     
         5 . The medical device of  claim 2 , wherein the polyether derivative is PEG. 
     
     
         6 . The medical device of  claim 1 , wherein the polyether derivative is covalently linked to an Fc region of the antibodies. 
     
     
         7 . The medical device of  claim 1 , wherein the medical device is a stent, an artificial valve, a vascular prosthetic filter, a catheter, a pacemaker, a vascular graft, a synthetic graft, a pacemaker lead, a defibrillator, a patent foramen ovale (PFO) septal closure device, a vascular clip, a vascular aneurysm occluder, a hemodialysis graft, a hemodialysis catheter, an atrioventricular shunt, an aortic aneurysm graft device or components, an artificial venous valve, a shunt, a wire, a sensor, a suture, a vascular anastomosis clip, an indwelling venous or arterial catheter, a vascular sheath or a drug delivery port. 
     
     
         8 . The medical device of  claim 1 , wherein the medical device is a stent. 
     
     
         9 . The medical device of  claim 1 , wherein the medical device is an artificial heart valve, a vascular stent graft, a synthetic graft, or an artificial venous valve. 
     
     
         10 . The medical device of  claim 1 , wherein the medical device is an artificial aortic valve, an artificial pulmonary valve, an artificial mitral valve, or an artificial tricuspid valve. 
     
     
         11 . The medical device of  claim 1 , comprising metal, and/or an alloy. 
     
     
         12 . The medical device of  claim 1 , further comprising a biocompatible polymer selected from the group consisting of polytetrafluoroethylene (PTFE), expanded PTFE (ePTFE), polyethylene terephthalate, polyethylene, polyurethane, polypropylene, combinations thereof, and derivatives thereof. 
     
     
         13 . The medical device of  claim 1 , wherein the coating further comprises a pharmaceutical substance. 
     
     
         14 . The medical device of  claim 13 , wherein the pharmaceutical substance inhibits smooth muscle cell migration and/or proliferation. 
     
     
         15 . The medical device of  claim 13 , wherein the pharmaceutical substance is paclitaxel, rapamycin, a rapamycin derivative, sirolimus, everolimus, tacrolimus, biolimus, biolimus A-9, or combinations thereof. 
     
     
         16 . The medical device of  claim 1 , wherein the antibodies and/or antibody fragments are humanized antibodies or antibody fragments, or chimeric antibodies or antibody fragments. 
     
     
         17 . The medical device of  claim 1 , wherein the antibodies and/or antibody fragments comprise Fab, F(ab′) 2 , or single chain Fv (scFv). 
     
     
         18 . The medical device of  claim 1 , wherein the antibodies and/or antibody fragments capture endothelial progenitor cells and/or endothelial cells in vivo when the medical device is implanted into a subject. 
     
     
         19 . A method for treating or preventing a vascular condition in a patient, the method comprising implanting the medical device of  claim 1  into the patient. 
     
     
         20 . The method of  claim 19 , wherein the vascular condition is atherosclerosis, restenosis, thrombosis, or blood vessel occlusion.