Formulation for improving physical endurance in athletes and a method for preparing the same

Abstract

The present disclosure discloses a herbal formulation in powdered dosage form, which is useful to enhance the physical endurance or strength in athletes. The disclosure also includes a method of preparation of the formulation, which involves the encapsulation of the natural ingredients in a natural matrix. The formulation comprises Moringa oleifera, Kaempferia parviflora and Punica granatum that are processed to obtain the product in powder form by encapsulation of the natural ingredients in a natural matrix. The formulation increased the nitrate and nitrite levels in serum and saliva in human and is useful to improve the general health of the athletes.

1 . A method of preparation of the formulation for improvement of physical endurance, the method comprising:
 a. crushing of  Moringa oleifera  leaf to obtain the powder and extracting the active constituents niazimin A and niazirin using a solvent;   b. crushing  Kaempferia parviflora  raw material to obtain the powder and extracting the active constituent 5, 7 dimethoxy flavone using ethanol;   c. mixing  Kaempferia parviflora  extract and  Moringa oleifera  extract together to form a uniform mixture;   d. crushing the dried peel of  Punica granatum  and extracting the active constituent punicalagin using water and subjecting to spray drying to obtain powder;   e. mixing  Punica granatum  dried peel extract with uniform mixture of  Kaempferia parviflora  extract and  Moringa oleifera  extract;   f. drying the uniform mixture of all the three extracts  Punica granatum  dried peel extract,  Kaempferia parviflora  extract and  Moringa oleifera  extract and blending and homogenizing to obtain a uniform matrix; and   g. spray drying the uniform matrix of the three herbal extracts to convert into powder form.   
     
     
         2 . The method as claimed in  claim 1 , wherein niazimin A and niazirin are extracted from  Moringa oleifera  leaf using hydro alcohol. 
     
     
         3 . The method as claimed in  claim 1 , wherein the formulation increases pre-exercise levels of nitrate, nitrite in serum and saliva and dopamine in human. 
     
     
         4 . The method as claimed in  claim 1 , wherein the formulation decreases levels of post-exercise lactate dehydrogenase and malondialdehyde in human. 
     
     
         5 . The method as claimed in  claim 1 , wherein the formulation promotes post exercise muscle recovery and reduction of oxidative stress in muscles.