US2021348239A1PendingUtilityA1

Detecting colorectal neoplasm

Assignee: MAYO FOUND MEDICAL EDUCATION & RESPriority: Mar 31, 2014Filed: Jul 16, 2021Published: Nov 11, 2021

Est. expiryMar 31, 2034(~7.7 yrs left)· nominal 20-yr term from priority

Inventors:David A. Ahlquist William R. Taylor Douglas W. Mahoney Graham P. Lidgard Hatim T. Allawi

C12Q 2600/154C12Q 2600/112C12Q 1/6886

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Claims

Abstract

Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as colorectal cancer.

Claims

exact text as granted — not AI-modified

We claim: 
     
         1 . A method comprising:
 extracting genomic DNA from a stool sample of a human individual suspected of having or having colorectal cancer,   treating the extracted genomic DNA with bisulfite,   amplifying the bisulfite-treated genomic DNA using primers specific for a CpG site for LRRC4, primers specific for a CpG site for LASS4, and primers specific for a CpG site for PPP2R5C,   measuring a methylation level of the CpG site for LRRC4, the CpG site for LASS4 and the CpG site for PPP2R5C.

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