Nanoreactor using polyion complex polymersomes, and method for producing same

Abstract

The present invention provides a nanoreactor (nano size reaction field) using a polyion complex polymersome and a method for producing the nanoreactor. The present invention provides a polyion complex polymersome encapsulating e.g., an enzyme, in which the enzyme is an enzyme acting on a substance passing through a membrane of the polyion complex polymersome, as a substrate.

1 . (canceled) 
     
     
         2 : A method of administering an enzyme having a molecular weight of 5 kDa or more, comprising:
 administering to a subject a polyion complex polymersome encapsulating the enzyme,   wherein the polyion complex polymersome comprises a polymer of formula (I),   
       
         
           
           
               
               
           
         
       
       where R1 is a hydroxyl group, a protecting group, a hydrophobic group or a polymerizable group, R4 is H, a protecting group, a hydrophobic group or a polymerizable group, R3 is a group represented by —(NH—(CH 2 ) 2 ) p —NH 2 , p is an integer of 1 to 5, and n is an integer of 0 to 5000 and m is an integer of 0 to 5000, provided that m+n is an integer of 2 to 5000, and that n−m is an integer of 0 or more. 
     
     
         3 : The method of  claim 2 , wherein the polymer of the polyion complex polymersome includes polymers crosslinked between the polymers. 
     
     
         4 : The method of  claim 2 , wherein in measurement in an aqueous solution of a 10 mM phosphate buffer solution having pH7.4 at 37° C., a cumulative release rate of the enzyme encapsulated 7 days after contact with the aqueous solution is 20% or less. 
     
     
         5 : The method of  claim 2 , wherein the enzyme is an enzyme that acts on a substrate in blood. 
     
     
         6 : The method of  claim 2 , wherein the enzyme is an enzyme that acts on a substrate in blood, and the polymer of the polyion complex polymersome includes polymers crosslinked between the polymers. 
     
     
         7 : The method of  claim 2 , wherein the polymer of the polyion complex polymersome includes polymers crosslinked between the polymers, and in measurement in an aqueous solution of a 10 mM phosphate buffer solution having pH7.4 at 37° C., a cumulative release rate of the enzyme encapsulated 7 days after contact with the aqueous solution is 20% or less. 
     
     
         8 : The method of  claim 2 , wherein the polymer of the polyion complex polymersome includes polymers crosslinked between the polymers, in measurement in an aqueous solution of a 10 mM phosphate buffer solution having pH7.4 at 37° C., a cumulative release rate of the enzyme encapsulated 7 days after contact with the aqueous solution is 20% or less, and the enzyme is an enzyme that acts on a substrate in blood. 
     
     
         9 : A method of administering an enzyme having a molecular weight of 5 kDa or more, comprising:
 administering to a subject a polyion complex polymersome comprising a polymer and encapsulating the enzyme,   wherein the polymer of the polyion complex polymersome includes polymers crosslinked between the polymers.   
     
     
         10 : The method of  claim 9 , wherein in measurement in an aqueous solution of a 10 mM phosphate buffer solution having pH7.4 at 37° C., a cumulative release rate of the enzyme encapsulated 7 days after contact with the aqueous solution is 20% or less. 
     
     
         11 : The method of  claim 9 , wherein in measurement in an aqueous solution of a 10 mM phosphate buffer solution having pH7.4 at 37° C., a cumulative release rate of the enzyme encapsulated 7 days after contact with the aqueous solution is 20% or less, and the enzyme is an enzyme that acts on a substrate in blood. 
     
     
         12 : The method of  claim 5 , wherein the substrate passes through membrane of the polyion complex polymersome. 
     
     
         13 : The method of  claim 6 , wherein the substrate passes through membrane of the polyion complex polymersome. 
     
     
         14 : The method of  claim 8 , wherein the substrate passes through membrane of the polyion complex polymersome. 
     
     
         15 : The method of  claim 11 , wherein the substrate passes through membrane of the polyion complex polymersome.