Composition for injection which can be used for treatment of heart diseases and contains fibroblasts, and method for producing fibroblast for therapy use

Abstract

The present invention aims to provide a method which has not been established yet and which is useful for achieving long-term and fundamental cure of a necrotic cardiac tissue region to allow recovery of functionality of the heart.The present invention provides an injectable composition for treatment of a cardiac disease, the composition comprising fibroblasts, wherein the fibroblasts contain CD106-positive fibroblasts, preferably contain CD90-positive fibroblasts, and the fibroblasts do not form colonies.

1 . A method of administering fibroblasts to a cardiac tissue in a subject, the method comprising:
 providing an injectable pharmaceutical composition comprising fibroblasts, the fibroblasts comprising CD106-positive fibroblasts wherein the ratio in terms of the cell number of the CD106-positive fibroblasts to the total amount of cells contained in the composition is not less than 70%; and   administering the injectable pharmaceutical composition to the subject by injection of the composition into the cardiac tissue and/or infusion of the composition into a coronary artery.   
     
     
         2 . The method of administering fibroblasts to a cardiac tissue in a subject according to  claim 1 , wherein the ratio in terms of the cell number of the CD106-positive fibroblasts to the total amount of cells contained in the composition is not less than 80%. 
     
     
         3 . The method of administering fibroblasts to a cardiac tissue in a subject according to  claim 1 , wherein a method of the injection comprises needle injection or needle free injection. 
     
     
         4 . The method of administering fibroblasts to a cardiac tissue in a subject according to  claim 1 , wherein a method of the infusion comprises using a catheter. 
     
     
         5 . The method of administering fibroblasts to a cardiac tissue in a subject according to  claim 1 , wherein the number of CD 106+ fibroblasts contained in the composition is not less than 1.0×10 2  cell/mL. 
     
     
         6 . The method of administering fibroblasts to a cardiac tissue in a subject according to  claim 1 , wherein the number of CD 106+ fibroblasts contained in the composition is not less than 1.0×10 4  cell/mL. 
     
     
         7 . The method of administering fibroblasts to a cardiac tissue in a subject according to  claim 1 , wherein the number of CD 106+ fibroblasts contained in the composition is not less than 5.0×10 5  cell. 
     
     
         8 . The method of administering fibroblasts to a cardiac tissue in a subject according to  claim 1 , wherein the injectable pharmaceutical composition is administered at several positions in the cardiac tissue by injection.