Novel aav8 mutant capsids and compositions containing same

Abstract

Provided herein are AAV8 mutant capsids and rAAV comprising the same. In one embodiment, vectors employing the AAV8 mutant capsid show increased transduction in a selected tissue as compared to AAV8.

What is claimed is: 
     
         1 . An adeno-associated virus comprising a capsid having the sequence of SEQ ID NO: 18 (AAV3G1), SEQ ID NO: 20 (AAV8.T20); or SEQ ID NO: 22 (AAV8.TR1). 
     
     
         2 . A nucleic acid encoding the capsid according to  claim 1 . 
     
     
         3 . The AAV according to  claim 1 , wherein the capsid is encoded by SEQ ID NO: 17, SEQ ID NO: 19 or SEQ ID NO: 21, or a sequence sharing at least 80% identity therewith. 
     
     
         4 . A recombinant adeno-associated virus (AAV) comprising an AAV capsid having an amino acid sequence selected from: SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 and 34, further comprising a non-AAV nucleic acid sequence. 
     
     
         5 . A nucleic acid molecule comprising a nucleic acid sequence encoding an AAV capsid protein, wherein said nucleic acid sequence is selected from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31 and 33.