Inhibition and treatment of gastrointestinal biofilms

Abstract

Orally administered physiologically acceptable anti-biofilm compositions comprising enzymes and if desired additional components such as antimicrobials, antibiotics, antifungals, herbals, chelating agents, lactoferrin and related compounds, minerals, surfactants, binders, and fillers useful for the inhibition and treatment of gastrointestinal biofilms in humans. Physiologically acceptable anti-biofilm compositions containing these enzymes are useful in the inhibition, reduction and/or treatment of gastrointestinal biofilm infections, and associated systemic symptoms caused by biofilms associated microorganisms within the gastrointestinal tract. Methods of identification, preparation and use of such physiologically acceptable anti-biofilm compositions are also provided.

1 . A method of inhibiting a gastrointestinal biofilm infection in a mammal, the method comprising:
 identifying the presence of the gastrointestinal biofilm infection; and   orally administering to the mammal a therapeutically effective amount of a composition comprising:
 a multi-enzyme mixture comprising: cellulase; glucoamylase; hemicellulase/pectinase complex; beta-glucanase; an enzyme with dipeptidyl-peptidase IV (DPP-IV) activity; chitosanase; lysozyme; a  Serratia  peptidase; and at least one pharmaceutically acceptable carrier, 
   in an amount and for a time sufficient to cause significant biofilm degradation within the gastrointestinal system of the mammal.   
     
     
         2 . The method of  claim 1 , wherein the amount of cellulase per dose ranges from 1 to 10,000 CU, the amount of hemicellulase/pectinase complex ranges from 1 to 8,000 HSU, and the amount of beta-glucanase ranges from 1 to 1000 BGU.