Identification of resistance genes from wild relatives of banana and their uses in controlling panama disease

Abstract

The present disclosure provides compositions and methods for providing broad-based resistance to fungal pathogens, such as a Fusarium fungi, and plants derived therefrom.

1 .- 38 . (canceled) 
     
     
         39 . A nucleic acid construct comprising a nucleic acid sequence coding for resistance to  Fusarium oxysporum  race 4 when expressed in a plant, wherein said nucleic acid sequence is selected from the group consisting of a nucleic acid sequence having at least 95% a sequence identity to SEQ ID NO: 2, SEQ ID NO: 5, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 18, SEQ ID NO: 21, and SEQ ID NO: 24, and wherein the nucleic acid sequence is operably linked to a promoter capable of driving expression of the nucleic acid sequence. 
     
     
         40 . The nucleic acid construct of  claim 39 , wherein the promoter is a plant promoter. 
     
     
         41 . The nucleic acid construct of  claim 39 , wherein the promoter is a 35S promoter. 
     
     
         42 . The nucleic acid construct of  claim 39 , wherein the promoter is coded by SEQ ID NO: 31. 
     
     
         43 . A transgenic plant, plant part, plant cell, or plant tissue culture comprising a nucleic acid construct comprising a nucleic acid sequence coding for resistance to  Fusarium oxysporum  race 4 when expressed in a plant, wherein said nucleic acid sequence is selected from the group consisting of a nucleic acid sequence having at least 95% sequence identity to SEQ ID NO: 2, SEQ ID NO: 5, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 18, SEQ ID NO: 21, and SEQ ID No: 24, and wherein the nucleic acid sequence is operably linked to a promoter capable of driving expression of the nucleic acid sequence. 
     
     
         44 . A method of transforming a plant cell comprising introducing the nucleic acid construct of  claim 39  into a plant cell, whereby the transformed plant cell expresses the nucleic acid sequence coding for resistance to  Fusarium oxysporum  race 4. 
     
     
         45 . The method of  claim 44 , wherein the plant cell is a  Musa  plant cell. 
     
     
         46 . The method of  claim 44 , wherein the plant cell is a  Musa acuminata  plant cell. 
     
     
         47 . The method of  claim 44 , further comprising producing a transformed plant tissue from the transformed plant cell. 
     
     
         48 . The method of  claim 47 , further comprising producing a transformed plantlet from the transformed plant tissue. 
     
     
         49 . The method of  claim 48 , further comprising producing a clone of the transformed plantlet. 
     
     
         50 . The method of  claim 48 , further comprising growing the transformed plantlet of the transformed plantlet into a mature transformed plant. 
     
     
         51 . The method of  claim 50 , wherein the mature transformed plant is a  Musa  plant and the mature transformed  Musa  plant is capable of producing fruit. 
     
     
         52 . The method of  claim 51 , further comprising producing clones of the mature transformed  Musa  plant. 
     
     
         53 . The method of  claim 51 , further comprising using the mature transformed  Musa  plant of the mature transformed  Musa  plant in a breeding method. 
     
     
         54 .- 77 . (canceled) 
     
     
         78 . The transgenic plant of  claim 43 , wherein the promoter is a plant promoter. 
     
     
         79 . The transgenic plant of  claim 43 , wherein the promoter is a 35S promoter. 
     
     
         80 . The transgenic plant of  claim 43 , wherein the promoter is coded by SEQ ID NO: 31.