Nsp-interleukin-10 proteins and uses thereof

Abstract

Disclosed herein are Nsp-IL10 polypeptides comprising an Nsp polypeptide and an IL10 polypeptide. In some embodiments, Nsp-IL10 polypeptide is capable of activating an NGF signaling pathway, an IL10 signaling pathway, or both. Also disclosed are methods for treating a disease comprising administering an Nsp-IL10 polypeptide. The methods include treating diseases associated with joint inflammation, such as osteoarthritis.

1 . An Nsp-IL10 polypeptide comprising an Nsp polypeptide and an IL10 polypeptide. 
     
     
         2 . The Nsp-IL10 polypeptide of  claim 1 , wherein the Nsp polypeptide and the IL10 polypeptide are directly linked. 
     
     
         3 . The Nsp-IL10 polypeptide of  claim 1 , wherein the Nsp polypeptide comprises SEQ ID NO: 2. 
     
     
         4 . The Nsp-IL10 polypeptide of  claim 1 , wherein the IL10 polypeptide comprises SEQ ID NO: 4. 
     
     
         5 . The Nsp-IL10 polypeptide of  claim 1 , further comprising a signal peptide. 
     
     
         6 . The Nsp-IL10 polypeptide of  claim 5 , wherein the signal peptide comprises SEQ ID NO: 5. 
     
     
         7 . The Nsp-IL10 polypeptide of  claim 1 , further comprising one or more linkers. 
     
     
         8 . The Nsp-IL10 polypeptide of  claim 7 , where the one linker is between the Nsp polypeptide and the IL10 polypeptide. 
     
     
         9 . The Nsp-IL10 polypeptide of  claim 7 , wherein the linker comprises SEQ ID NO: 6. 
     
     
         10 . The Nsp-IL10 polypeptide of  claim 1 , wherein the IL10 polypeptide comprises an amino acid sequence having at least 60% identity with SEQ ID NO: 4. 
     
     
         11 . The Nsp-IL10 polypeptide of  claim 1 , wherein the polypeptide comprises SEQ ID NO: 7. 
     
     
         12 . A polynucleotide encoding the polypeptide of  claim 1 . 
     
     
         13 .- 19 . (canceled) 
     
     
         20 . A kit comprising a vector comprising a polynucleotide sequence encoding an Nsp-IL10 polynucleotide operably linked to a promoter, wherein the Nsp-IL10 polynucleotide comprises an Nsp polynucleotide and an IL10 polynucleotide.