Oro-nasal Inhalation Plethysmography Mask Exposure System

Abstract

In at least one embodiment, a system includes an inhalation source; a plenum in fluid communication with the inhalation source; at least one challenge plethysmography mask in fluid communication with the plenum, for each challenge plethysmography mask including a mask having a cavity to receive at least a nose of a test animal, a delivery conduit in fluid communication with the plenum and the cavity of the mask, at least one exhaust conduit in fluid communication with the cavity of the mask and the plenum, and a pressure sensor attached to the mask to measure pressure within the cavity of the mask; at least one processor in electrical communication with the at least one pressure sensor, the processor configured to process an output signal of the pressure sensor into respiratory data for each test animal during an exposure study; and an exhaust system in fluid communication with the plenum.

1 . A system comprising:
 an inhalation source;   a plenum in fluid communication with said inhalation source;   at least one challenge plethysmography mask in fluid communication with said plenum, each challenge plethysmography mask including
 a mask having a cavity in which to receive at least a nose of a test animal, 
 a delivery conduit in fluid communication between said plenum and the cavity of said mask, 
 at least one exhaust conduit in fluid communication with the cavity of said mask and said plenum, and 
 a pressure sensor attached to said mask to measure pressure within the cavity of said mask; and 
   at least one processor in electrical communication with said at least one pressure sensor, said processor configured to process an output signal of said pressure sensor into respiratory data for each test animal during an exposure study.   
     
     
         2 . The system according to  claim 1 , further comprising:
 at least one sampling mask is in fluid communication with said plenum and in electrical communication with said at least one processor; and   an aerosol diluter/APS in fluid communication with said at least one sampling mask and in electrical communication with said at least one processor.   
     
     
         3 . The system according to  claim 2 , wherein said at least one sampling mask system further includes
 a concentration sampling mask in fluid communication with said plenum;   an aerosol sampler in fluid communication with said concentration sampling mask;   at least one filter in fluid communication with said aerosol sampler and said exhaust system.   
     
     
         4 . The system according to  claim 1 , wherein said inhalation source includes
 a compressed gas source;   a gas flow controller in fluid communication with said compressed gas source; and   a nebulizer in fluid communication with said gas flow controller and said plenum.   
     
     
         5 . The system according to  claim 4 , wherein said inhalation source further includes
 a second gas flow controller in fluid communication with said compressed gas source; and   a radial mixer in fluid communication with said nebulizer, said second gas flow controller and said nebulizer such that the gas flows from said nebulizer and said second gas flow controller are mixed together prior to discharge towards said plenum.   
     
     
         6 . The system according to  claim 1 , wherein said inhalation source further includes a diffusion dryer in fluid communication with said plenum such that the gas flow from said inhalation source passes through said diffusion dryer prior to entry into said plenum. 
     
     
         7 . The system according to  claim 1 , wherein said exhaust system includes
 at least one filter in fluid communication with said plenum;   an exhaust gas flow controller in fluid communication with said plenum such that said exhaust gas flow controller regulates the flow rate exiting said plenum; and   a vacuum in fluid communication with said exhaust gas flow controller to draw the exiting gas flow from said plenum.   
     
     
         8 . The system according to  claim 1 , further comprising an exhaust sink having a reservoir to at least one of kill organic inhalants and neutralize organic inhalants. 
     
     
         9 . The system according to  claim 1 , wherein each challenge plethysmography mask having two exhaust conduits attached on opposing surfaces of said mask. 
     
     
         10 . The system according to  claim 9 , wherein said two exhaust conduits from a Y-shaped conduit such that said stem of the Y is in fluid communication with said plenum. 
     
     
         11 . The system according to  claim 1 , wherein said plenum includes
 a housing having a cavity in fluid communication with said inhalation source and said exhaust system; and   a plurality of connection ports in fluid communication with and configured to attach to any of said delivery conduit and said exhaust conduit to establish fluid communication from said plenum cavity to said connected delivery conduit or exhaust conduit.   
     
     
         12 . The system according to  claim 1 , wherein said inhalation source includes
 a compressed gas source;   a gas flow controller in fluid communication with said compressed gas source; and   a generator configured to add an inhalant material into the gas flow to create an aerosol.   
     
     
         13 . A challenge plethysmography mask for use in an exposure system, said challenge plethysmography mask comprising:
 a mask having an open-ended cavity in which to receive a snout of a test animal,   a delivery conduit connectable to a plenum and in fluid communication with the cavity of said mask,   at least one exhaust conduit in fluid communication with the cavity of said mask and connectable to the plenum, and   a pressure sensor attached to said mask to measure pressure within the cavity of said mask.   
     
     
         14 . The challenge plethysmography mask according to  claim 13 , wherein said two exhaust conduits from a Y-shaped conduit such that said stem of the Y is in fluid communication with said plenum. 
     
     
         15 . A method for performing an exposure study of at least one test animal using an exposure system, the method comprising:
 placing the snout of one test animal into a challenge plethysmography mask having a delivery conduit connecting the mask to a plenum, at least one exhaust conduit connecting the mask to the plenum, and a pressure sensor on the mask to measure a pressure within the cavity of the mask;   creating an environment within the plenum having material provided by an inhalation source where the material is being used in the exposure study;   passing the environment through the delivery conduit to the mask;   passing the environment in the mask including expiration from the test animal through the at least one exhaust conduit to the plenum;   sending a pressure signal from the pressure sensor to a processor;   sampling characteristics of the environment present in the plenum; and   calculating, with the processor connected to the pressure sensor, a dosage received by the test animal based on respiration data obtained from the pressure signal provided by the pressure sensor and environment characteristics.   
     
     
         16 . The method according to  claim 15 , wherein sampling characteristics is done intermittently on a predetermined schedule. 
     
     
         17 . The method according to  claim 15 , wherein sampling characteristics includes sampling a concentration of a material being used in the exposure study. 
     
     
         18 . The method according to  claim 15 , wherein calculating the dosage includes
 calculating the respiratory frequency for the test animal based on the pressure signal from the pressure sensor,   calculating a minute volume based on the respiratory frequency and a tidal volume,   correlating the characterization data for the environment with the respiratory information, and   determining the dosage received by the test animal.   
     
     
         19 . The method according to  claim 15 , wherein the characterization data includes particle size for the material. 
     
     
         20 . The method according to  claim 15 , further comprising:
 creating the environment using a nebulizer attached to a compressed gas source; and   pulling the environment from the plenum using a vacuum.