Genetically-modified poultry egg

Abstract

Provided are a poultry knock-in egg and knock-out egg. The present invention pertains to a knock-out poultry egg in which at least one oviduct-specific gene has been knocked out, said gene being selected from the group consisting of ovalbumin, ovomucoid, ovomucin, ovotransferrin, ovoinhibitor, and lysozyme, and at least one egg allergen protein has been reduced or eliminated, said protein being selected from the group consisting of ovalbumin, ovomucoid, ovomucin, ovotransferrin, ovoinhibitor, and lysozyme.

1 - 22 . (canceled) 
     
     
         23 . A genetically modified chicken egg whose genome comprises a deletion, a substitution, or an insertion of a base or bases in a coding region of an endogenous ovomucoid gene, wherein the chicken egg that does not functionally express endogenous ovomucoid. 
     
     
         24 . The chicken egg of  claim 23 , wherein the coding region is exon 3 of ovomucoid gene. 
     
     
         25 . The chicken egg of  claim 24 , wherein the coding region is OVMtg2 represented by the nucleotide sequence of SEQ ID NO: 6. 
     
     
         26 . A genetically modified chicken whose genome comprises a deletion, a substitution, or an insertion of a base or bases in a coding region of an endogenous ovomucoid gene, wherein the chicken is capable of laying an egg that does not functionally express endogenous ovomucoid. 
     
     
         27 . The chicken of  claim 26 , wherein the coding region is exon 3 of ovomucoid gene. 
     
     
         28 . The chicken egg of  claim 27 , wherein the coding region is OVMtg2 represented by the nucleotide sequence of SEQ ID NO: 6. 
     
     
         29 . A method of producing a genetically modified knock-out chicken egg, the method comprising:
 a) deleting, substituting, or inserting a base or bases in a coding region of an endogenous ovomucoid gene of an isolated chicken primordial germ cell (PGC), wherein:
 the endogenous ovomucoid gene is inactivated; 
   b) transplanting the PGC obtained in step a) into a recipient chicken embryo;   c) producing a genetically modified knock-out male chicken and a genetically modified knock-out female chicken from the chicken embryo in step b);   d) mating the genetically modified knock-out male chicken and the genetically modified knock-out female chicken;   e) obtaining an offspring knock-out chicken, wherein the offspring knock-out chicken is capable of laying an egg; and   f) obtaining a genetically modified knock-out chicken egg from the knock-out offspring chicken of step e), wherein the chicken egg does not functionally express endogenous ovomucoid.   
     
     
         30 . The method of  claim 29 , wherein the coding region is exon 3 of ovomucoid gene. 
     
     
         31 . The method of  claim 30 , wherein the coding region is OVMtg2 represented by the nucleotide sequence of SEQ ID NO: 6. 
     
     
         32 . The method of  claim 29 , wherein the base or bases in the coding region of the endogenous ovomucoid gene in step a) is deleted, substituted, or inserted using CRISPR and guide RNA.