Pharmaceutical composition for dermatology and uses thereof

Abstract

The present invention refers to a composition comprising a conditioned cell culture medium obtained or obtainable by a process which comprises culturing a population of mesenchymal stromal cells (MSCs), in which at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99%, of said population by number of cells are MSCs obtained from a mammal pertaining to the genus Canis , in a nutrient rich liquid prepared for cell culture, preferably a basal media; and collecting the conditioned cell culture medium, wherein preferably the nutrient rich liquid is an animal/human serum-free media or a chemically defined animal/human serum-free and xeno free media designed to grow MSCs, for use in therapy.

1 . A method of xenogenically treating psoriasis in a human subject, the method comprising administering to the human subject having psoriasis an amount of a composition comprising a conditioned cell culture medium effective for xenogenic treatment of psoriasis in the human subject, the conditioned cell culture medium made by a process comprising:
 culturing a population of mesenchymal stromal cells (MSCs) or immortalized cells obtained therefrom, in which at least 50% of said population by number of cells are MSCs obtained from a mammal of the genus  Canis  or immortalized cells obtained therefrom, in a nutrient rich liquid or a basal media suitable for propagating the MSCs; and   collecting the conditioned cell culture medium,   
     
     
         2 . The method according to  claim 1 , wherein the MSCs are obtained from a dog species. 
     
     
         3 . The method according to  claim 1 , wherein the MSCs are umbilical-cord derived stromal cells, adipose tissue-derived stromal cells, expanded mesenchymal stromal cells, expanded adipose tissue-derived stromal cells, bone-marrow derived stromal cells, expanded bone-marrow derived stromal cells, or immortalized mesenchymal stromal cells obtained therefrom. 
     
     
         4 . The method according to  claim 1 , wherein the nutrient rich liquid prepared for cell culture is a buffered saline solution comprising amino acids and vitamins supplemented with sodium pyruvate and glutamine. 
     
     
         5 . The method of  claim 1 , wherein the nutrient rich liquid is a basal media with supplementation. 
     
     
         6 . The method of  claim 1 , wherein such composition is formulated to deliver an amount of conditioned medium at an appropriate interval to effectively treat psoriasis. 
     
     
         7 . The method according to  claim 6 , wherein such composition is formulated for administration by an intravenous, oral, or topical route. 
     
     
         8 . The method of  claim 7 , wherein such route of administration is topical, and wherein the composition is a topical formulation that is formulated in liquid or in semi-solid form. 
     
     
         9 . The method of  claim 7 , wherein such route of administration is topical, and wherein the composition is a topical formulation that is formulated in a form selected from: a liquid, a fluid, a foam, a cream, a gel, a paste, a balsam, a spray, an ointment, a lotion, a conditioner, a tonic, a milk, a mousse, an emulsion, a serum, an oil, a stick, a shampoo, a jelly, a suspension, a dispersion, a lacquer, a paint, an elixir, a drop and an aerosol form. 
     
     
         10 . The method of  claim 7 , wherein such route of administration is topical, and wherein the composition is a topical formulation that is formulated in a liposomal preparation.