METHOD FOR EFFICIENTLY PRODUCING ß MYOSIN HEAVY CHAIN IN CARDIAC MUSCLE CELLS DIFFERENTIATED FROM INDUCED PLURIPOTENT STEM CELLS DERIVED FROM HOMO SAPIENS

Abstract

The present invention provides a method for producing a β myosin heavy chain in cardiac muscle cells differentiated from induced pluripotent stem cells derived from Homo sapiens . In the present method, first, a liquid culture medium containing the cardiac muscle cells is supplied onto a substrate comprising a first electrode, a second electrode and insulative fibers on the surface thereof. At least a part of the insulative fibers is located between the first electrode and the second electrode in a top view of the substrate. Then, the substrate is left at rest. Finally, the cardiac muscle cells are cultivated, while a pulse electric current is applied to the cardiac muscle cells through the first electrode and the second electrode.

1 . A substrate comprising:
 a first electrode;   a second electrode; and   insulative fibers, wherein   the first electrode, the second electrode, and the insulative fibers are provided on a surface of the substrate;   at least a part of the insulative fibers is located between the first electrode and the second electrode in a top view of the substrate; and   an angle formed between each of not less than 90% of the insulative fibers and an imaginary straight line which passes through both the first electrode and the second electrode is not more than ±20 degrees in the top view.   
     
     
         2 . The substrate according to  claim 1 , further comprising
 a reference electrode on the surface thereof.