Enterobacter and its application

Abstract

The invention belongs to the field of cosmetic biotechnology, and specifically relates to an Enterobacter that degrades hyaluronic acid and a cultivation method and application thereof. The Enterobacter sp. CGJ001 of the present invention was deposited in the China General Microbiological Culture Collection Center on Oct. 10, 2019, and the preservation number is CGMCC NO. 18661. The Enterobacter strain can efficiently produce hyaluronidase, and can be used in the process of preparing low molecular hyaluronic acid and oligomeric hyaluronic acid from high molecular hyaluronic acid. The enzyme has high specificity towards hyaluronic acid, excellent thermal stability and pH stability, and is suitable for large-scale industrial application. Thus it can replace the traditional hyaluronidase extracted from expensive animal tissues. There should be broad application prospects in the fields of medicine and cosmetics.

What is claimed is: 
     
         1 . Enterobacter sp. CGJ001 deposited at the China General Microbiological Culture Collection Center on Oct. 10, 2019 at No. 3, Yard. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences under Accession Number CGMCC NO. 18661. 
     
     
         2 . The Enterobacter of  claim 1  wherein the Enterobacter CGJ001 has the effect of degrading hyaluronic acid. 
     
     
         3 . A method of producing hyaluronidase comprising subjecting the Enterobacter CGJ001 of  claim 1  to plate culture, seed culture, and fermentation culture. 
     
     
         4 . The method of  claim 3  comprising:
 (1) plate culturing the Enterobacter CGJ001 to obtain plate strains; 
 (2) inoculating the plate strains into sterilized seed culture medium and incubating at 30-40° C. and 150-300 rpm for 12-24 h to obtain seed liquid; 
 (3) inoculating the seed liquid into a sterilized fermentation medium and incubating at 30-40° C. and 150-300 rpm for 12-24 h to obtain a hyaluronidase-containing bacterial liquid. 
 
     
     
         5 . The method of  claim 4  wherein the seed medium and fermentation medium components comprise 1˜10 g/L hyaluronic acid, 1˜5 g/L K3PO4, 0.1˜1 g/L MgSO4, 1˜10 g/L L peptone, 1˜10 g/L yeast powder. 
     
     
         6 . The method of  claim 3  wherein the degradation product of hyaluronidase by the enzyme is 2-6 sugars.