US2021187509A1PendingUtilityA1

Method and analysis system for testing a sample

Assignee: BOEHRINGER INGELHEIM VETMEDICA GMBHPriority: Oct 7, 2016Filed: Mar 4, 2021Published: Jun 24, 2021
Est. expiryOct 7, 2036(~10.2 yrs left)· nominal 20-yr term from priority
B01L 2300/0819B01L 2300/0809B01L 2300/0883C12Q 1/686B01L 2200/147B01L 7/52B01L 2300/0636B01L 3/5027B01L 2300/0816B01L 2300/1827C12Q 1/6806B01L 2200/10B01L 2300/0645C12Q 1/6825B01L 2300/1822B01L 3/502761
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Claims

Abstract

An analysis system for testing, in particular, a biological sample, the sample and/or analytes of the sample being temperature-controlled in advance or denatured immediately before the test, and/or being heated, the heat being transferred through a chip of a sensor apparatus.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . An analysis system for testing a biological sample, comprising:
 at least one receiving cavity for the sample,   a plurality of reaction cavities for forming amplification products of analytes of the sample, and   a sensor apparatus for detecting at least one of the analytes or the amplification products,   wherein the reaction cavities are fluidically arranged between the at least one receiving cavity and the sensor apparatus,   wherein the sensor apparatus comprises a sensor compartment fluidically linked to the plurality of reaction cavities,   wherein the sensor apparatus comprises capture molecules for bonding at least one of the analytes or the amplification products, and   wherein the capture molecules have different hybridization temperatures at which the at least one of the analytes or the amplification products bonds to the corresponding capture molecules.   
     
     
         2 . The analysis system according to  claim 1 , further comprising a mixing cavity for mixing the sample with at least one reagent, wherein the mixing cavity is fluidically arranged between the at least one receiving cavity and the plurality of reaction cavities. 
     
     
         3 . The analysis system according to  claim 2 , wherein the mixing cavity is provided with at least one reagent. 
     
     
         4 . The analysis system according to  claim 1 , further comprising a plurality of groups of reagents for carrying out an amplification reaction for forming the amplification products. 
     
     
         5 . The analysis system according to  claim 4 , wherein a different group of the plurality of groups of reagents is assigned to each reaction cavity of the plurality of reaction cavities. 
     
     
         6 . The analysis system according to  claim 4 , wherein each group of the plurality of groups of reagents comprises a group of primers for carrying out the amplification reaction. 
     
     
         7 . The analysis system according to  claim 6 , wherein the groups of primers differ from one another such that amplification products with different hybridization temperatures can be formed in the plurality of reaction cavities. 
     
     
         8 . The analysis system according to  claim 4 , wherein the plurality of groups of reagents are arranged in the plurality of reaction cavities. 
     
     
         9 . The analysis system according to  claim 4 , the analysis system further comprising a plurality of intermediate cavities, wherein the plurality of groups of reagents are contained in the intermediate cavities. 
     
     
         10 . The analysis system according to  claim 9 , wherein the intermediate cavities are at least one of fluidically arranged in parallel to one another or between the plurality of reaction cavities and the at least one receiving cavity. 
     
     
         11 . The analysis system according to  claim 1 , wherein the reaction cavities are fluidically arranged in parallel to one another. 
     
     
         12 . The analysis system according to  claim 1 , further comprising an intermediate temperature-control cavity for actively temperature-controlling at least one of the analytes or amplification products, wherein the intermediate temperature-control cavity is fluidically arranged between the plurality of reaction cavities on one side and the sensor apparatus on another side. 
     
     
         13 . The analysis system according to  claim 12 , further comprising an intermediate temperature-control apparatus for actively temperature-controlling the intermediate temperature-control cavity. 
     
     
         14 . The analysis system according to  claim 12 , wherein the sensor apparatus is fluidically connected to all the of the reaction cavities via the intermediate temperature-control cavity. 
     
     
         15 . The analysis system according to  claim 1 , wherein the sensor apparatus comprises a support and a plurality of electrodes arranged on the support, wherein a sensor temperature-control apparatus is provided for directly temperature-controlling the support. 
     
     
         16 . The analysis system according to  claim 15 , wherein the support comprises a chip or is formed by a chip. 
     
     
         17 . The analysis system according to  claim 1 , further comprising a connection apparatus for at least one of electrically or thermally connecting the sensor apparatus with an analysis device. 
     
     
         18 . The analysis system according to  claim 17 , wherein the connection apparatus is movable against the sensor apparatus. 
     
     
         19 . The analysis system according to  claim 17 , wherein the connection apparatus comprises a sensor temperature-control apparatus for controlling the temperature of the sensor apparatus. 
     
     
         20 . The analysis system according to  claim 1 , further comprising a cartridge for receiving the sample and an analysis device for receiving the cartridge. 
     
     
         21 . Analysis system according to  claim 20 , wherein the cartridge comprises at least one of the at least one receiving cavity, the sensor apparatus or the plurality of reaction cavities. 
     
     
         22 . The analysis system according to  claim 20 , wherein the analysis device comprises at least one of a reaction temperature-control apparatus assigned to the plurality of reaction cavities, an intermediate temperature-control apparatus assigned to an intermediate temperature-control cavity, a sensor temperature-control apparatus for temperature-controlling the sensor apparatus or a connection apparatus for at least one of electrically or thermally connecting the sensor apparatus to the cartridge.

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