Multifunctional Treatment And Diagnostic Compositions And Methods

Abstract

Multifunctional compositions and methods are provided for therapeutic treatment of bacteria and cancers and for fluorescence diagnosis. Systems generate in situ reactive oxygen species such as singlet oxygen ( 1 O 2 ), hydroxyl radical (OH) and Juglone, and other chemotherapeutic agents. Methods provided selectively produce greater amounts of one reactive oxygen species over others. Variations are effective in aerobic, anaerobic or H 2 O 2 rich environments and in presence of, or absence of, light. In H 2 O 2 rich environment in absence of light, variations decompose H 2 O 2 into O 2 gas to remove excess H 2 O 2 for elimination of hypoxic environment. Variations are formed of porphyrins, naphthalene derivatives, and metal ions, for illustration, free base tetrakis Ar substituted porphyrine core without metal or halide substitution but having hydroxyphenyl and alkyl pyridyl substituents at meso positions combined with dihydroxynaphthalene and +3 hydrated metal ions.

What is claimed is: 
     
         1 - 9 . (canceled) 
     
     
         10 . A method to produce one or more reactive oxygen species and Juglone or its derivatives in situ as resultant effect of one or more variations of (ArPP+DHN+hMe(III)) and reaction product or other result of combination of ArPP, DHN, and hMe(III) wherein optionally mole ratio of hMe(III) is increased or decreased in relation to mole ratios of ArPP and DHN to selectively produce greater or lesser amount of one reactive oxygen species or Juglone or its derivatives over other resultant effects. 
     
     
         11 . A method to produce hydroxyl radicals in situ by forming (ArPP+DHN+hMe(III)) and reaction product or other result of combination of ArPP, DHN, and hMe(III) in presence of light or absence of light, wherein in absence of light and in anaerobic conditions, hydroxyl radical ({dot over (O)}H) is produced upon reacting with hydrogen peroxide (H 2 O 2 ). 
     
     
         12 . A method to produce hydroxyl radicals in situ in a condition which is aerobic, anaerobic, or H 2 O 2  rich environment or any sequence or combination of said conditions, by forming (ArPP+DHN+hMe(III)) and reaction product or other result of combination of ArPP, DHN, and hMe(III) in presence or absence of light wherein in absence of light and in anaerobic conditions, hydroxyl radical ({dot over (O)}H) is produced upon reacting combination of ArPP, DHN, and hMe(III) with hydrogen peroxide (H 2 O 2 ). 
     
     
         13 . A method to produce Juglone or Juglone derived chemotherapeutics in situ in a condition which is aerobic, anaerobic, or H 2 O 2  rich environment or any sequence or combination of said conditions, by forming (ArPP+DHN+hMe(III)) and reaction product or other result of combination of ArPP, DHN, and hMe(III) in presence or absence of light. 
     
     
         14 . A method to produce singlet oxygen in situ in a condition which is which is aerobic, anaerobic, or H 2 O 2  rich environment or any sequence or combination of said conditions by forming (ArPP+DHN+hMe(III)) and reaction product or other result of combination of ArPP, DHN, and hMe(III) in presence of light. 
     
     
         15 . A method of producing in presence of visible light and in aerobic condition one or more of singlet oxygen ( 1 O 2 ), hydroxyl radical ({dot over (O)}H), Juglone or Juglone derivatives by forming (ArPP+DHN+hMe(III)) and reaction product or other result of combination of ArPP, DHN, and hMe(III). 
     
     
         16 . A method of producing in absence of light and in anaerobic condition one or more of hydroxyl radical ({dot over (O)}H) derived from hydrogen peroxide (H 2 O 2 ), Juglone or Juglone derivatives by forming (ArPP+DHN+hMe(III)) and reaction product or other result of combination of ArPP, DHN, and hMe(III). 
     
     
         17 . A method of producing one or more non-toxic chemotherapeutic treatments by forming (ArPP+DHN+hMe(III)) and reaction product or other result of combination of ArPP, DHN, and hMe(III) by forming at or near mammalian tissue or fluid in one or more regions of treatment
 (a) in presence of visible light
 (1) in an aerobic condition singlet oxygen ( 1 O 2 ), hydroxyl radical ({dot over (O)}H), and one or more of Juglone or Juglone derivatives, then concurrently or sequentially 
 (2) in an anaerobic condition, hydroxyl radical ({dot over (O)}H) and Juglone or Juglone derivatives, 
   then forming either concurrently or subsequently forming at or near mammalian tissue or fluid in one or more different regions of treatment   (b) in absence of light,
 (1) hydroxyl radical ({dot over (O)}H) upon reacting ArPP, DHN, and hMe(III) with hydrogen peroxide (H 2 O 2 ) and one or more of Juglone or Juglone derivatives. 
   
     
     
         18 . A method to treat tumor hypoxia by oxygenating a less well-oxygenated necrotic region of a solid mammalian tumor having a wide range of oxygen concentrations not just at extremes of fully oxygenated or fully hypoxic, by forming (ArPP+DHN+hMe(III)) and reaction product or other result of combination of ArPP, DHN, and hMe(III) by adjusting concentration of hMe(III) ions combined with DHN and ArPP to enable control of the rate of oxidation of DHN by ArPP in the presence of selected amounts of hMe(III) ions to form a tailored treatment composition as non-toxic chemotherapeutic agent of choice by selectively activating one or more of resulting reaction products of singlet oxygen ( 1 O 2 ), hydroxyl radical ({dot over (O)}H), Juglone, or its derivatives as nontoxic reaction product or product of choice in in lieu of one or more of other reaction products. 
     
     
         19 . (canceled)