Methods and compositions for detection of zika viral infections

Abstract

Applicant discloses herein kits for identifying the presence of Zika virus in a sample. In embodiments, these kits comprise reagents disclosed herein. Applicant further provides kits for use in detecting ZIKV in a sample, the kits comprising reagents disclosed herein. In embodiments, kits include primers directed to Zika virus (ZIKV) nucleic acid sequences, the primers capable of hybridizing to ZIKV nucleic acids and to copies of ZIKV nucleic acids (including to cDNA copies of ZIKV nucleic acids). Applicant discloses herein reagents for detecting Zika virus (ZIKV) in a sample, the reagents including one or more nucleic acid primers that are capable of hybridizing to a ZIKV nucleic acid (including to cDNA copies of ZIKV nucleic acids).

1 - 18 . (canceled) 
     
     
         19 . A method for detecting a virus, the method comprising:
 providing a kit comprising: a sample process control reagent, a virus positive control reagent, a nucleic acid primer probe mixture,   using said kit with a blood sample obtained from a fingerstick to provide a processed sample; and   using a nucleic acid amplification process on the processed sample to determine a presence of the virus in the processed sample.   
     
     
         20 . The method of  claim 19  wherein the kit further comprises a buffer. 
     
     
         21 . The method of  claim 20 , wherein the buffer is selected from phosphate, tris(hydroxymethyl)aminomethane (TRIS), 3-(N-morpholino) propanesulfonic acid (MOPS), 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO), 2-(N-morpholino)ethanesulfonic acid (MES), N-(2-Acetamido)-iminodiacetic acid (ADA), and piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES). 
     
     
         22 . The kit of  claim 20 , wherein the buffer is tris(hydroxymethyl)aminomethane (TRIS). 
     
     
         23 . The method of  claim 19  further comprising a reporter molecule. 
     
     
         24 . The method of  claim 19  wherein the kit comprises a nucleic acid primer that comprises a reporter molecule. 
     
     
         25 . The method of  claim 23  wherein the reporter molecule comprises a fluorescent moiety, and the nucleic acid primer further comprises a quenching moiety effective to quench fluorescence from the fluorescent moiety when the primer is not hybridized to a target nucleic acid sequence.