Information processing system, mutation detection system, storage medium, and information processing method

Abstract

Provided is an information processing system including: a functionality prediction result acquisition unit configured to acquire a result of predicting a functionality of a test target gene in a sequence of a test genome, the test target gene having a sequence different from a reference genome; and a determination unit configured to determine an introduction of an artificial mutation based on the result acquired by the functionality prediction result acquisition unit.

What is claimed is: 
     
         1 . An information processing system comprising:
 at least one memory storing instructions; and   at least one processor configured to execute the instructions to:   acquire a result of predicting a functionality of a test target gene in a sequence of a test genome, the test target gene having a sequence different from a reference genome; and   determine an introduction of an artificial mutation based on the result acquired.   
     
     
         2 . The information processing system according to  claim 1 , wherein the at least one processor is further configured to execute the instructions to acquire a result of identifying, in the sequence including the test target gene, a mutation introduction portion including a PAM sequence and a target sequence which are usable in editing using a CRISPR-Cas9 system. 
     
     
         3 . The information processing system according to  claim 2 , wherein the at least one processor is further configured to execute the instructions to extract, from the sequence of the test genome, a mutation introduction site which has a sequence different from the reference genome and which includes the PAM sequence and the target sequence when a sequence different from the reference genome in the sequence of the test genome is present in the target sequence in the result acquired. 
     
     
         4 . The information processing system according to  claim 1 , wherein the reference genome is a genome of a parent strain of an individual having the test genome. 
     
     
         5 . The information processing system according to  claim 1 , wherein the reference genome is a genome of a tissue which is of an individual having the test genome and which is different from a tissue having the test genome. 
     
     
         6 . The information processing system according to  claim 1 , wherein the reference genome is a genome which is obtained from the same tissue as a tissue having the test genome and which is obtained before the test genome. 
     
     
         7 . A mutation detection system comprising:
 a genome purification device configured to extract and purify a genome from a cell or a virus;   a sequencer configured to determine a sequence of the genome obtained by the genome purification unit; and   the information processing system of  claim 1 .   
     
     
         8 . A non-transitory storage medium having stored thereon an information processing program for causing a computer to:
 acquire a result of predicting a functionality of a sequence of a test target gene in a sequence of a test genome, the sequence of the test target gene having a sequence different from a reference genome; and   determine an introduction of an artificial mutation based on the result of predicting the functionality.   
     
     
         9 . The non-transitory storage medium having stored thereon an information processing program according to  claim 8 , wherein the information processing program further causes the computer to acquire a result of identifying, in the sequence including the test target gene, a mutation introduction portion including a PAM sequence and a target sequence which are usable in editing using a CRISPR-Cas9 system. 
     
     
         10 . The non-transitory storage medium having stored thereon an information processing program according to  claim 9 , wherein the information processing program further causes the computer to extract, from the sequence of the test genome, a mutation introduction site which has a sequence different from the reference genome and which includes the PAM sequence and the target sequence when a sequence different from the reference genome in the sequence of the test genome is present in the target sequence in the result of identifying the mutation introduction portion. 
     
     
         11 . An information processing method comprising:
 a functionality prediction result acquisition step of acquiring a result of predicting a functionality of a test target gene in a sequence of a test genome, the test target gene having a sequence different from a reference genome; and   a step of determining an introduction of an artificial mutation based on the result acquired in the functionality prediction result acquisition step.   
     
     
         12 . The information processing method according to  claim 11 , further comprising a mutation introduction portion identification step of acquiring a result of identifying, in the sequence including the test target gene, a mutation introduction portion including a PAM sequence and a target sequence which are usable in editing using a CRISPR-Cas9 system. 
     
     
         13 . The information processing method according to  claim 12 , further comprising a mutation introduction site extraction step of extracting, from the sequence of the test genome, a mutation introduction site which has a sequence different from the reference genome and which includes the PAM sequence and the target sequence when a sequence different from the reference genome in the sequence of the test genome is present in the target sequence in the result acquired in the mutation introduction portion identification step.