Systems and methods for growing cells in vitro

Abstract

A system for growing cells comprising a bioreactor chamber for growing the cells, a delivery system delivering a perfusion solution to the bioreactor chamber for perfusion of the perfusion solution through the cells, a dialysis system having a dialyzer, a dialysate for performing a dialysis and a filter for reducing ammonia content in said dialysate, and a controller that circulates the perfusion solution through the dialyzer and the dialysate through the filter.

What is claimed is: 
     
         1 . A method of generating a composition comprising a cultured fat and a plant-derived protein matrix, the method comprising:
 culturing an adipocyte cell in vitro to obtain the cultured fat; and   adding the plant-derived protein matrix to the cultured fat,   
       thereby generating the composition comprising cultured fat and a plant-derived protein matrix. 
     
     
         2 . The method according to  claim 1 , wherein the adipocyte cell is generated from a fibroblast. 
     
     
         3 . The method of  claim 2 , wherein the fibroblast is a spontaneously immortalized fibroblast. 
     
     
         4 . The method according to  claim 1 , wherein the culturing of the adipocyte cell occurs in a suspension culture. 
     
     
         5 . The method of according to  claim 1 , wherein the culturing of the adipocyte cell is performed on a plant-derived protein matrix, thereby generating the cultured fat on the plant-derived protein matrix. 
     
     
         6 . The method according to  claim 3 , further comprising generating the adipocyte cell from a spontaneously immortalized fibroblast by culturing a spontaneously immortalized fibroblast in a serum-free medium comprising oleic acid and a peroxisome proliferator-activated receptor gamma (PPAR-gamma) agonist or activator. 
     
     
         7 . The method of  claim 6 , wherein the PPAR-gamma agonist or activator is rosiglitazone. 
     
     
         8 . The method of  claim 6 , wherein the spontaneously immortalized fibroblast is a chicken embryonic fibroblast. 
     
     
         9 . The method according to  claim 1 , wherein the plant-derived protein matrix comprises at least one plant-derived protein from the legume (Fabaceae) family, the cereal family, or the pseudocereal family. 
     
     
         10 . The method according to  claim 9 , wherein the at least one plant-derived protein is from the legume (Fabaceae) family and is selected from the group consisting of alfalfa, pea, bean, lentil, carob, soybean, and peanut proteins and combinations thereof. 
     
     
         11 . The method according to  claim 10 , wherein the at least one plant-derived protein is a soy protein or a pea protein. 
     
     
         12 . The method according to  claim 9 , wherein the at least one plant-derived protein is from the cereal family and is selected from the group consisting of maize, rice, wheat, barely, sorghum, millet, oats, rye, tritcale, and fonio proteins and combinations thereof. 
     
     
         13 . The method according to  claim 9 , wherein the at least one plant-derived protein is from the pseudocereal family and is selected from the group consisting of buckwheat,  quinoa , and chia proteins and combinations thereof. 
     
     
         14 . The method according to  claim 1 , wherein the plant-derived protein matrix comprises a soy protein or a pea protein. 
     
     
         15 . A composition produced according to the method of  claim 1 , wherein the composition is a meat substitute product. 
     
     
         16 . A meat substitute product comprising:
 a cultured fat obtained from culturing, in-vitro, an adipocyte cell; and   a plant-derived protein matrix.   
     
     
         17 . The meat substitute product of  claim 16 , wherein the adipocyte cell is generated from a fibroblast. 
     
     
         18 . The meat substitute product of  claim 17 , wherein the fibroblast is a spontaneously immortalized fibroblast. 
     
     
         19 . The meat substitute product of  claim 16 , wherein the adipocyte cell is cultured in a suspension medium. 
     
     
         20 . The meat substitute product of  claim 19 , wherein the suspension medium is a serum-free medium comprising oleic acid and a peroxisome proliferator-activated receptor gamma (PPAR-gamma) agonist or activator. 
     
     
         21 . The meat substitute product of  claim 16 , wherein the plant-derived protein matrix comprises at least one plant-derived protein from the legume (Fabaceae) family, the cereal family, or the pseudocereal family. 
     
     
         22 . The meat substitute product of  claim 21 , wherein the at least one plant-derived protein is from the legume (Fabaceae) family and is selected from the group consisting of alfalfa, pea, bean, lentils, carob, soybean, and peanut proteins and combinations thereof. 
     
     
         23 . The meat substitute product of  claim 22 , wherein the at least one plant-derived protein is a soy protein or a pea protein. 
     
     
         24 . The meat substitute product of  claim 21 , wherein the at least one plant-derived protein is from cereal family and is selected from the group consisting of maize, rice, wheat, barely, sorghum, millet, oats, rye, tritcale, and fonio proteins and combinations thereof. 
     
     
         25 . The meat substitute product of  claim 22 , wherein the at least one plant-derived protein is from the pseudocereal family and is selected from the group consisting of buckwheat, quinoa, and chia proteins and combinations thereof. 
     
     
         26 . The meat substitute product of  claim 16 , wherein the plant-derived protein matrix comprises a soy protein or a pea protein. 
     
     
         27 . The meat substitute product of  claim 16 , wherein the spontaneously immortalized fibroblast is a chicken embryonic fibroblast. 
     
     
         28 . A composition for the in-vitro production of cultured fat, the composition comprising
 an adipocyte cell; and   a serum-free medium,   wherein the adipocyte cell generates the cultured fat.   
     
     
         29 . The composition of  claim 28 , wherein the adipocyte cell is generated from a spontaneously immortalized fibroblast. 
     
     
         30 . The composition of  claim 29 , wherein the serum free medium comprises oleic acid and PPAR-gamma agonist or activator.