Humanized anti-bag3 antibodies

Abstract

The present invention provides for a humanized anti-BAG3 antibody or a fragment thereof, pharmaceutical compositions comprising said antibody and its use as a medicament, in particular for use in the treatment of pancreatic tumours or other pathologies of an immune, inflammatory, neoplastic, cardiovascular and/or degenerative nature.

1 . A humanized antibody or a fragment thereof which binds to the BAG3 protein, comprising:
 a) a heavy chain having the amino acid sequence of SEQ ID NO: 12 or an amino acid sequence having at least 95% sequence identity thereof,   b) a light chain having the amino acid sequence of SEQ ID NO: 20 or an amino acid sequence having at least 95% sequence identity thereof.   
     
     
         2 . The humanized antibody or a fragment thereof according to  claim 1 , which is a Fab fragment, a Fab′ fragment, a F(ab′) fragment, a Fv fragment, a diabody, a ScFv, a small modular immunopharmaceutical (SMIP), an affibody, an avimer, a nanobody, a domain antibody, or single chains. 
     
     
         3 . A nucleic acid encoding the antibody or a fragment thereof according to  claim 1 . 
     
     
         4 . A vector comprising the nucleic acid according to  claim 3 . 
     
     
         5 . A host cell comprising the nucleic acid of  claim 3 . 
     
     
         6 . A pharmaceutical composition comprising at least one antibody or a fragment thereof according to  claim 1  and at least one pharmaceutically acceptable excipient or carrier. 
     
     
         7 . The pharmaceutical composition according to  claim 6 , in a form of oral formulation, parenteral formulation, or topical administration. 
     
     
         8 . The pharmaceutical composition according to  claim 6 , further comprising antimetabolites, camptothecins, or taxanes. 
     
     
         9 . A method for treating a pathological state which involves the activation of macrophages, selected from the group consisting of: neoplastic diseases, inflammatory diseases, immune diseases and degenerative diseases, comprising administering the humanized antibody or a fragment thereof according to  claim 1  to a subject in need thereof. 
     
     
         10 . The method according to  claim 9 , for treating pancreatic tumor or bladder tumor.