Fc VARIANTS WITH ALTERED BINDING TO FcRn

Abstract

The present application relates to optimized IgG immunoglobulin variants, engineering methods for their generation, and their application, particularly for therapeutic purposes.

1 .- 24 . (canceled) 
     
     
         25 . A protein comprising an Fc variant as compared to a parent IgG Fc region, said variant Fc region comprising an amino acid substitution selected from the group consisting of Q311V, H285D and N286D, wherein numbering is according to the EU index in Kabat et al. 
     
     
         26 . An antibody comprising an Fc variant as compared to a parent IgG Fc region, said variant Fc region comprising an amino acid substitution selected from the group consisting of Q311V, H285D and N286D, wherein numbering is according to the EU index in Kabat et al. 
     
     
         27 . A nucleic acid encoding the protein of  claim 25 . 
     
     
         28 . A nucleic acid encoding the heavy chain of said antibody of  claim 26 . 
     
     
         29 . An expression vector comprising the nucleic acid of  claim 27 . 
     
     
         30 . A host cell comprising the expression vector of  claim 29 . 
     
     
         31 . A method of making a protein comprising an Fc variant as compared to a parent IgG Fc region, said variant Fc region comprising an amino acid substitution selected from the group consisting of Q311V, H285D and N286D, wherein numbering is according to the EU index in Kabat et al., said method comprising culturing the host cell of  claim 30  under conditions wherein said antibody is expressed and recovering said antibody.