High Affinity Human Antibodies to Human Angiopoietin-2

Abstract

The present invention provides antibodies that bind to angiopoietin-2 (Ang-2) and methods of using same. According to certain embodiments of the invention, the antibodies are fully human antibodies that bind to human Ang-2. The antibodies of the invention are useful, inter alia, for the treatment of diseases and disorders associated with one or more Ang-2 biological activities including angiogenesis.

1 - 24 . (canceled) 
     
     
         25 . An isolated nucleic acid comprising a nucleotide sequence that encodes a heavy chain variable region comprising a HCDR1, HCDR2 and HCDR3 of a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 18; and a light chain variable region comprising a LCDR1, LCDR2 and LCDR3 of a light chain variable region comprising the amino acid sequence of SEQ ID NO: 20. 
     
     
         26 . The nucleic acid of  claim 25 , wherein:
 the HCDR1 comprises the amino acid sequence set forth in SEQ ID NO: 4,   the HCDR2 comprises the amino acid sequence set forth in SEQ ID NO: 6,   the HCDR3 comprises the amino acid sequence set forth in SEQ ID NO: 8,   the LCDR1 comprises the amino acid sequence set forth in SEQ ID NO: 12,   the LCDR2 comprises the amino acid sequence set forth in SEQ ID NO: 14, and   the LCDR3 comprises the amino acid sequence set forth in SEQ ID NO: 16.   
     
     
         27 . The nucleic acid of  claim 26 , wherein:
 the nucleic acid encoding the HCDR1 comprises the nucleotide sequence set forth in SEQ ID NO: 3,   the nucleic acid encoding the HCDR2 comprises the nucleotide sequence set forth in SEQ ID NO: 5,   the nucleic acid encoding the HCDR3 comprises the nucleotide sequence set forth in SEQ ID NO: 7,   the nucleic acid encoding the LCDR1 comprises the nucleotide sequence set forth in SEQ ID NO: 11,   the nucleic acid encoding the LCDR2 comprises the nucleotide sequence set forth in SEQ ID NO: 13,   the nucleic acid encoding the LCDR3 comprises the nucleotide sequence set forth in SEQ ID NO: 15.   
     
     
         28 . The nucleic acid of  claim 25 , wherein the heavy chain variable region comprises the amino acid sequence set forth in SEQ ID NO: 18; and the light chain variable region comprises the amino acid sequence set forth in SEQ ID NO: 20. 
     
     
         29 . The nucleic acid of  claim 28 , wherein the nucleic acid encoding the heavy chain variable region comprises the nucleotide sequence set forth in SEQ ID NO: 17; and the nucleic acid encoding the light chain variable region comprises the nucleotide sequence set forth in SEQ ID NO: 19. 
     
     
         30 . A recombinant expression vector comprising the nucleic acid of  claim 25 . 
     
     
         31 . A recombinant expression vector comprising the nucleic acid of  claim 26 . 
     
     
         32 . A recombinant expression vector comprising the nucleic acid of  claim 27 . 
     
     
         33 . A recombinant expression vector comprising the nucleic acid of  claim 28 . 
     
     
         34 . A recombinant expression vector comprising the nucleic acid of  claim 29 . 
     
     
         35 . A host cell comprising the nucleic acid of  claim 25 . 
     
     
         36 . A host cell comprising the nucleic acid of  claim 26 . 
     
     
         37 . A host cell comprising the nucleic acid of  claim 27 . 
     
     
         38 . A host cell comprising the nucleic acid of  claim 28 . 
     
     
         39 . A host cell comprising the nucleic acid of  claim 29 . 
     
     
         40 . A method for producing an antibody or antigen-binding fragment thereof comprising introducing the recombinant expression vector of  claim 30  into a host cell, culturing the host cell under conditions permitting production of the antibody or fragment, and recovering the antibody or fragment produced. 
     
     
         41 . A method for producing an antibody or antigen-binding fragment thereof comprising introducing the recombinant expression vector of  claim 31  into a host cell, culturing the host cell under conditions permitting production of the antibody or fragment, and recovering the antibody or fragment produced. 
     
     
         42 . A method for producing an antibody or antigen-binding fragment thereof comprising introducing the recombinant expression vector of  claim 32  into a host cell, culturing the host cell under conditions permitting production of the antibody or fragment, and recovering the antibody or fragment produced. 
     
     
         43 . A method for producing an antibody or antigen-binding fragment thereof comprising introducing the recombinant expression vector of  claim 33  into a host cell, culturing the host cell under conditions permitting production of the antibody or fragment, and recovering the antibody or fragment produced. 
     
     
         44 . A method for producing an antibody or antigen-binding fragment thereof comprising introducing the recombinant expression vector of  claim 34  into a host cell, culturing the host cell under conditions permitting production of the antibody or fragment, and recovering the antibody or fragment produced.