Ultra bright dimeric or polymeric dyes and methods for preparation of the same

Abstract

Dimeric and/or polymeric dyes and compounds and methods for preparation of the same are disclosed.

1 .- 70 . (canceled) 
     
     
         71 . A composition, comprising:
 a first dye oligomer comprising:
 a first compound comprising a first plurality of fluorescent moieties, the first compound having a first terminal and a second terminal; 
 a first nucleic acid molecule having a first sequence, the first nucleic acid molecule being covalently bound to the first terminal; 
 a second nucleic acid molecule having a second sequence, the second nucleic acid molecule being covalently bound to the second terminal; and 
   a second dye oligomer comprising:
 a second compound comprising a second plurality of fluorescent moieties, the second compound having a third terminal and a fourth terminal; 
 a third nucleic acid molecule having a third sequence that is complementary to the second sequence, the third nucleic acid molecule being covalently bound to the third terminal, the third nucleic acid molecule being hybridized to the second nucleic acid molecule; 
 a fourth nucleic acid molecule having a fourth sequence, the fourth nucleic acid molecule being covalently bound to the fourth terminal. 
   
     
     
         72 . The composition of  claim 71 , further comprising:
 a third dye oligomer comprising:
 a fourth compound comprising a fourth plurality of fluorescent moieties, the fourth compound having a fifth terminal and a sixth terminal; 
 a fifth nucleic acid molecule having a fifth sequence that is complementary to the fourth sequence, the fifth nucleic acid molecule being covalently bound to the fifth terminal, the fifth nucleic acid molecule being hybridized to the fourth nucleic acid molecule; 
 a sixth nucleic acid molecule having a sixth sequence, the sixth nucleic acid molecule being covalently bound to the sixth terminal. 
   
     
     
         73 . The composition of  claim 71 , wherein the first and the third sequences are the same. 
     
     
         74 . The composition of  claim 73 , wherein the first dye oligomer and the second dye oligomer are the same. 
     
     
         75 . The composition of  claim 74 , wherein the composition comprises a plurality of dye oligomers that are the same, the plurality of dye oligomers comprising the first dye oligomer and the second dye oligomer. 
     
     
         76 . A composition, comprising:
 a nucleic acid-polymer composite comprising:
 a polymer backbone 
 a first nucleic acid molecule covalently bound to the polymer backbone, the first nucleic acid molecule having a first sequence; and 
   a second nucleic acid molecule having a second sequence that is complementary to the first sequence, the second nucleic acid molecule being covalently bound to a first oligomer dye comprising a first plurality of fluorescent moieties, the first nucleic acid molecule being hybridized to the second nucleic acid molecule.   
     
     
         77 . The composition of  claim 76 , wherein the nucleic acid-polymer composite further comprises a third nucleic acid molecule covalently bound to the polymer backbone, the third nucleic acid molecule having a third sequence. 
     
     
         78 . The composition of  claim 77 , further comprising a fourth nucleic acid molecule having a fourth sequence that is complementary to the third sequence, the fourth nucleic acid molecule being covalently bound to a second oligomer dye comprising a second plurality of fluorescent moieties, the third nucleic acid molecule being hybridized to the fourth nucleic acid molecule 
     
     
         79 . The composition of  claim 78 , wherein the first sequence and the third sequence are the same. 
     
     
         80 . The composition of  claim 78 , wherein the first oligomer dye and the second oligomer dye are the same. 
     
     
         81 . A method, comprising:
 providing a first dye oligomer comprising:
 a first compound comprising a first plurality of fluorescent moieties, the first compound having a first terminal and a second terminal; 
 a first nucleic acid molecule having a first sequence, the first nucleic acid molecule being covalently bound to the first terminal; 
 a second nucleic acid molecule having a second sequence, the second nucleic acid molecule being covalently bound to the second terminal; and 
   contacting the first dye oligomer with a second dye oligomer comprising:
 a second compound comprising a second plurality of fluorescent moieties, the second compound having a third terminal and a fourth terminal; 
 a third nucleic acid molecule having a third sequence that is complementary to the second sequence, the third nucleic acid molecule being covalently bound to the third terminal; 
 a fourth nucleic acid molecule having a fourth sequence, the fourth nucleic acid molecule being covalently bound to the fourth terminal, 
   wherein the second nucleic acid molecule hybridizes to the third nucleic acid molecule.   
     
     
         82 . The method of  claim 81 , further comprising:
 contacting the second dye oligomer with a third dye oligomer comprising:
 a fourth compound comprising a fourth plurality of fluorescent moieties, the fourth compound having a fifth terminal and a sixth terminal; 
 a fifth nucleic acid molecule having a fifth sequence that is complementary to the fourth sequence, the fifth nucleic acid molecule being covalently bound to the fifth terminal; 
 a sixth nucleic acid molecule having a sixth sequence, the sixth nucleic acid molecule being covalently bound to the sixth terminal, 
   wherein the fourth nucleic acid molecule hybridizes to the fifth nucleic acid molecule.   
     
     
         83 . The method of  claim 81 , wherein the first dye oligomer and the second dye oligomer are the same. 
     
     
         84 . The method of  claim 81 , wherein the first and the third sequences are the same. 
     
     
         85 . A method, comprising:
 providing a nucleic acid-polymer composite comprising a polymer backbone covalently bound to a first nucleic acid molecule having a first sequence; and   contacting the first nucleic acid molecule with a second nucleic acid molecule having a second sequence that is complementary to the first sequence, the second nucleic acid molecule being covalently bound to a first oligomer dye comprising a first plurality of fluorescent moieties, thereby hybridizing the first nucleic acid molecule and the second nucleic acid molecule.   
     
     
         86 . The method of  claim 85 , wherein the nucleic acid-polymer composite further comprises a third nucleic acid molecule covalently bound to the polymer backbone, the third nucleic acid molecule having a third sequence. 
     
     
         87 . The method of  claim 86 , further comprising contacting the third nucleic acid molecule with a fourth nucleic acid molecule having a fourth sequence that is complementary to the third sequence, the fourth nucleic acid molecule being covalently bound to a second oligomer dye comprising a second plurality of fluorescent moieties, the third nucleic acid molecule hybridizing to the fourth nucleic acid molecule 
     
     
         88 . The method of  claim 87 , wherein the first sequence and the third sequence are the same. 
     
     
         89 . The method of  claim 87 , wherein the first oligomer dye and the second oligomer dye are the same.