Three dimensional tissue printing device, three dimensional tissue printing method and artificial skin

Abstract

A three dimensional tissue printing method is disclosed. The three dimensional tissue printing method includes the following steps: performing large support stand printing to form a first printing body; performing small support stand printing to form second printing body on the first printing body and forming a tissue structure by crossly connecting in between the first printing body and the second printing body. Besides, a three dimensional tissue printing device and artificial skin are also presented.

What is claimed is: 
     
         1 . A three dimensional tissue printing method, comprising the following steps:
 performing a large support stand printing to form a first printing body; and   performing small support stand printing to form a second printing body on the first printing body;   wherein, a tissue structure is formed by crossly connecting in between the first printing body and the second printing body.   
     
     
         2 . The three dimensional tissue printing method as claimed in  claim 1 , further comprising:
 performing a cell printing to instill a plurality of cells on the tissue structure formed by crossly connecting in between the first printing body and the second printing body.   
     
     
         3 . The three dimensional tissue printing method as claimed in  claim 2 , wherein the step of performing cell printing further comprising stirring the plurality of cells. 
     
     
         4 . The three dimensional tissue printing method as claimed in  claim 2 , wherein the plurality of cells are the human-body fiber mother cell. 
     
     
         5 . The three dimensional tissue printing method as claimed in  claim 1 , wherein the step of performing the large support stand printing further comprising the following steps:
 providing a temperature-reaction type material;   cooling the temperature-reaction type material;   squeezing out the temperature-reaction type material; and   curing the temperature-reaction type material.   
     
     
         6 . The three dimensional tissue printing method as claimed in  claim 5 , wherein the temperature-reaction type material can be a collagen, a melt-state material, a flow-state polylactide (PLA) with dissolved-solution added, or a polycaprolactone (PCL). 
     
     
         7 . The three dimensional tissue printing method as claimed in  claim 5 , wherein the step of cooling the temperature-reaction type material includes making the temperature-reaction type material appear flow-state. 
     
     
         8 . The three dimensional tissue printing method as claimed in  claim 5 , wherein in the step of curing the temperature-reaction type material includes making the temperature-reaction type material appear quasi-plastic state. 
     
     
         9 . The three dimensional tissue printing method as claimed in  claim 1 , wherein performing the small support stand printing further comprising the following steps:
 exerting an electric voltage;   making a material of the small support stand printing generate static charge accumulation; and   forming a micro jet stream under the traction of the static charge.   
     
     
         10 . The three dimensional tissue printing method as claimed in  claim 9 , wherein the material is a volatile macromolecule material, the flow-state polylactide (PLA) with dissolved-solution added or polycaprolactone (PCA). 
     
     
         11 . The three dimensional tissue printing method as claimed in  claim 9 , wherein the material is a solid state drawing snag, powders, or granular state, and through the process of stirring, melting, or heat melting. 
     
     
         12 . An artificial skin, comprising:
 a first printing body, constituted by a temperature-reaction type material;   a second printing body, forming a tissue structure by crossly connecting in between the first printing body and the second printing body; and   a plurality of human-body fiber mother cells positioned at the tissue structure formed by crossly connecting in between the first printing body and the second printing body.   
     
     
         13 . The artificial skin as claimed in  claim 12 , wherein the temperature-reaction type material is a collagen, a melt-state material, a flow-state polylactide (PLA) with dissolved-solution added, or a polycaprolactone (PCL). 
     
     
         14 . The artificial skin as claimed in  claim 12 , wherein the material of the second printing body is a volatile macromolecule material, a flow-state polylactide (PLA) with dissolved-solution added or polycaprolactone (PCL). 
     
     
         15 . The artificial skin as claimed in  claim 12 , wherein the material of the second printing body is a flow-state material processed by having a solid state drawing snag, powders, or granular state, process by stirring, melting, or heat melting.