Tissue-specific differentiation matrices and uses thereof

Abstract

In some aspects, this invention provides a method of making a bone marrow-derived tissue-specific stem cell proliferation, expansion, isolation and rejuvenation extracellular matrix. In other aspects, this invention provides a method of making a tissue-specific fibroblast-derived stem cell differentiation extracellular matrix. Also provided are methods of using such a cell-derived preservation or differentiation matrices to induce tissue-specific differentiation of pluripotent cells, repair damaged tissue, and treat a subject having a physiologic deficiency using the same.

1 .- 48 . (canceled) 
     
     
         49 . A method of treating a subject having a physiologic deficiency comprising:
 a) contacting a sample containing MSCs with a rejuvenating matrix to produce rejuvenated MSCs; and   b) introducing the rejuvenated MSCs into the subject,   wherein the physiologic deficiency is treated.   
     
     
         50 . The method of  claim 49 , wherein the sample is a human or murine sample. 
     
     
         51 . The method of  claim 49 , wherein the rejuvenating matrix is a preservation matrix generated by bone marrow cells obtained from a subject that is younger than the subject having a physiologic deficiency. 
     
     
         52 . The method of  claim 51 , wherein the subject is an elderly subject suffering from one or more of osteopenia, osteoporosis, sarcopenia and cachexia. 
     
     
         53 . The method of  claim 49 , wherein the rejuvenating matrix is a 3D rejuvenating matrix. 
     
     
         54 .- 106 . (canceled)