Antigen-specific helper t-cell receptor genes

Abstract

The present invention pertains to polynucleotides that encode CDR3 in TCR-[alpha] and TCR-[beta] chain genes of CD4+ helper T-cells that are specific to WT1 helper peptides having an amino acid sequence represented by SEQ ID NO: 123. The present invention further pertains to the peptides encoded by said polynucleotides. The present invention further pertains to CD4+ T cells into which TCR genes that contain said polynucleotides have been introduced, the induction of WT1-specific cytotoxic T-lymphocytes (CTLs) using the CD4+ T-cells, the treatment of cancer, etc.

1 - 22 . (canceled) 
     
     
         23 . An αCDR3 peptide encoded by a polynucleotide having a nucleotide sequence selected from the group consisting of SEQ ID NOs: 1, 3, 5, 8, 10, 11, 13, 14, 16, 18, 20, 22, 23, 25, 27, 28, 30, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 56, and 58, wherein said polynucleotide encodes CDR3 of α-chain of TCR of a CDR +  helper T-cell specific to a WT1 helper peptide (WT1 332  peptide) comprising SEQ ID NO: 123 or a sequence at least 90% identical to SEQ ID NO: 123. 
     
     
         24 . A βCDR3 peptide encoded by a polynucleotide having a nucleotide sequence selected from the group consisting of SEQ ID NOs: 2, 4, 6, 7, 9, 12, 15, 17, 19, 21, 24, 26, 29, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 57, and 59, wherein said polynucleotide encodes CDR3 of β-chain of TCR of a CD4 +  helper T-cell specific to WT1 332  peptide. 
     
     
         25 . A pair of peptides encoded by a pair of polynucleotides chosen from:
 SEQ ID NO:1 and SEQ ID NO:2;   SEQ ID NO:3 and SEQ ID NO:4;   SEQ ID NO:5 and SEQ ID NO:6;   SEQ ID NO:3 and SEQ ID NO:7;   SEQ ID NO:8 and SEQ ID NO:9;   SEQ ID NO:10 and SEQ ID NO:12;   SEQ ID NO:11 and SEQ ID NO:12;   SEQ ID NO:13 and SEQ ID NO:15;   SEQ ID NO:14 and SEQ ID NO:15   SEQ ID NO:16 and SEQ ID NO:17   SEQ ID NO:18 and SEQ ID NO:19   SEQ ID NO:20 and SEQ ID NO:21   SEQ ID NO:22 and SEQ ID NO:24   SEQ ID NO:23 and SEQ ID NO:24   SEQ ID NO:25 and SEQ ID NO:26   SEQ ID NO:27 and SEQ ID NO:4   SEQ ID NO:28 and SEQ ID NO:29   SEQ ID NO:30 and SEQ ID NO:32   SEQ ID NO:31 and SEQ ID NO:32   SEQ ID NO:33 and SEQ ID NO:34   SEQ ID NO:35 and SEQ ID NO:36   SEQ ID NO:37 and SEQ ID NO:38   SEQ ID NO:39 and SEQ ID NO:40   SEQ ID NO:41 and SEQ ID NO:42   SEQ ID NO:43 and SEQ ID NO:44   SEQ ID NO:45 and SEQ ID NO:46   SEQ ID NO:47 and SEQ ID NO:48   SEQ ID NO:49 and SEQ ID NO:50   SEQ ID NO:51 and SEQ ID NO:52   SEQ ID NO:53 and SEQ ID NO:54   SEQ ID NO:55 and SEQ ID NO:57   SEQ ID NO:56 and SEQ ID NO:57; or   SEQ ID NO:58 and SEQ ID NO:59   
       wherein each of the polynucleotide sequences may be a complementary sequence or a degenerate sequence thereof. 
     
     
         26 . A chip comprising the peptide according to  claim 23  or  24 , or the pair of the peptides according to  claim 25 .