Nutritional supplements and methods of nutritional supplementation affecting microbiome metabolism

Abstract

Nutritional supplements and methods of nutritional supplementation are described for improving microbiome balance in a subject. The methods include administering to a subject an effective amount of a composition including a probiotic including at least one species of probiotic bacterium, a prebiotic including at mixture of at least one of isomaltooligosaccharide, galactooligosaccharide and/or galactomannan, and a phytonutrient including a mixture of at least one of apple fruit extract, grapeseed extract, and/or pine bark extract.

1 . A method of improving microbiome balance in a subject, the method comprising:
 administering to the subject an effective amount of a composition comprising:
 a probiotic comprising at least one species of probiotic bacterium; 
 a prebiotic comprising at mixture of at least one of isomaltooligosaccharide, galactooligosaccharide and/or galactomannan; and 
 a phytonutrient comprising a mixture of at least one of apple fruit extract, grapeseed extract, and/or pine bark extract. 
   
     
     
         2 . The method of  claim 1 , wherein the probiotic comprises at least one of  Lactobacillus rhamnosus  R0011,  Bifidobacterium longum  R0175, and  Lactobacillus helveticus  R0052. 
     
     
         3 . The method of  claim 2 , wherein the probiotic comprises a mixture of at least two of  Lactobacillus rhamnosus  R0011,  Bifidobacterium longum  R0175, and  Lactobacillus helveticus  R0052. 
     
     
         4 . The method of  claim 1 , wherein the prebiotic comprises at least two of isomaltooligosaccharide, galactooligosaccharide and/or galactomannan. 
     
     
         5 . The method of  claim 4 , wherein the prebiotic comprises at least about 5 grams of at least two of isomaltooligosaccharide, galactooligosaccharide and/or galactomannan. 
     
     
         6 . The method of  claim 1 , wherein the phytonutrient comprises at least two of apple fruit extract, grapeseed extract, and/or pine bark extract. 
     
     
         7 . The method of  claim 1 , further comprising the step of identifying a subject in need of improvement of microbiome balance. 
     
     
         8 . The method of  claim 7 , wherein identifying a subject in need of treatment includes identifying a subject in need of at least one of body fat reduction, blood cholesterol reduction, blood triglyceride reduction, cortisol reduction, mood improvement, and/or increases in beneficial gut bacterial species. 
     
     
         9 . A method of improving microbiome balance in a subject, the method comprising:
 administering to the subject an effective amount of a composition comprising:
 a probiotic mixture comprising at least two of  Lactobacillus rhamnosus  R0011,  Bifidobacterium longum  R0175, and  Lactobacillus helveticus  R0052; 
 a prebiotic comprising at mixture of at least one of isomaltooligosaccharide, galactooligosaccharide and/or galactomannan; and 
 a phytonutrient comprising a mixture of at least one of apple fruit extract, grapeseed extract, and/or pine bark extract. 
   
     
     
         10 . The method of  claim 9 , wherein the probiotic comprises a mixture of  Lactobacillus rhamnosus  R0011,  Bifidobacterium longum  R0175, and  Lactobacillus helveticus  R0052. 
     
     
         11 . The method of  claim 9 , wherein the prebiotic comprises a mixture of isomaltooligosaccharide, galactooligosaccharide and galactomannan. 
     
     
         12 . The method of  claim 9 , wherein the phytonutrient comprises a mixture of apple fruit extract, grapeseed extract, and/or pine bark extract. 
     
     
         13 . The method of  claim 9 , further comprising the step of identifying a subject in need of improvement of microbiome balance. 
     
     
         14 . The method of  claim 13 , wherein identifying a subject in need of treatment includes identifying a subject in need of at least one of body fat reduction, blood cholesterol reduction, blood triglyceride reduction, cortisol reduction, mood improvement, and/or increases in beneficial gut bacterial species. 
     
     
         15 . (canceled) 
     
     
         16 . A composition, comprising:
 a probiotic comprising at least one species of probiotic bacterium;   a prebiotic comprising at mixture of at least one of isomaltooligosaccharide, galactooligosaccharide and/or galactomannan; and   a phytonutrient comprising a mixture of at least one of apple fruit extract, grapeseed extract, and/or pine bark extract.   
     
     
         17 . The composition of  claim 16 , wherein the probiotic comprises at least one of  Lactobacillus rhamnosus, Bifidobacterium longum,  and  Lactobacillus helveticus.    
     
     
         18 . The method of  claim 17 , wherein the  Lactobacillus rhamnosus  is  Lactobacillus rhamnosus  R0011. 
     
     
         19 . The composition of  claim 17 , wherein the  Bifidobacterium longum  is  Bifidobacterium longum  R0175. 
     
     
         20 . The composition of  claim 17 , wherein the  Lactobacillus helveticus  is  Lactobacillus helveticus  R0052. 
     
     
         21 . The method of  claim 16 , wherein the probiotic comprises a mixture of at least two of  Lactobacillus rhamnosus  R0011,  Bifidobacterium longum  R0175, and  Lactobacillus helveticus  R0052. 
     
     
         22 . The method of  claim 16 , wherein the prebiotic comprises at least two of isomaltooligosaccharide, galactooligosaccharide and/or galactomannan. 
     
     
         23 . The method of  claim 22 , wherein the prebiotic comprises at least about 5 grams of at least two of isomaltooligosaccharide, galactooligosaccharide and/or galactomannan. 
     
     
         24 . The method of  claim 16 , wherein the phytonutrient comprises at least two of apple fruit extract, grapeseed extract, and/or pine bark extract. 
     
     
         25 - 33 . (canceled)