Tomato pathogenic fungus detecting apparatus and detecting method using same

Abstract

The present disclosure provides a simple and secure apparatus and a simple and secure method for selectively detecting a tomato pathogenic fungus. The tomato pathogenic fungus detecting apparatus according to the present disclosure is characterized by including an artificial cell wall, a test sample solution inlet provided above the artificial cell wall, and a culture solution storage part provided under the artificial cell wall, wherein a test sample solution contains a 50 mM to 70 mM buffer solution of a citrate salt in the test sample solution inlet, and the test sample solution has a pH of 5 to 5.5.

1 . A tomato pathogenic fungus detecting apparatus comprising:
 an artificial cell wall;   a test sample solution inlet provided above the artificial cell wall; and   a culture solution storage part provided under the artificial cell wall,   wherein   a test sample solution contains a 50 mM to 70 mM buffer solution of a citrate salt in the test sample solution inlet; and   the test sample solution has a pH of 5 to 5.5.   
     
     
         2 . The tomato pathogenic fungus detecting apparatus according to  claim 1 , wherein
 the artificial cell wall includes a substrate that has a through hole with a hole diameter of 2 μm to 7 μm and has a thickness of 5 μm to 150 μm, and a cellulose membrane that is provided on one surface of the substrate and has a thickness of 0.5 μm to 2 μm.   
     
     
         3 . The tomato pathogenic fungus detecting apparatus according to  claim 1 , wherein
 the citrate salt is at least one selected from the group consisting of sodium citrate and potassium citrate.   
     
     
         4 . The tomato pathogenic fungus detecting apparatus according to  claim 1 , wherein
 a tomato pathogenic fungus to be a detection target is at least one selected from the group consisting of tomato gray mold fungus  Botrytis cinerea , tomato sooty mold fungus  Pseudocercospora fuligena , and a tomato leaf mold fungus  Passalora fulva.      
     
     
         5 . The tomato pathogenic fungus detecting apparatus according to  claim 1 , wherein
 the tomato pathogenic fungus detecting apparatus does not detect a  Biscogniauxia  genus fungus, a  Penicillium  genus fungus, a  Phoma  genus fungus, and a  Trichoderma  genus fungus, these four of which are tomato non-pathogenic fungi.   
     
     
         6 . The tomato pathogenic fungus detecting apparatus according to  claim 5 , wherein
 the  Biscogniauxia  genus fungus is  Biscogniauxia maritima,      the  Penicillium  genus fungus is  Penicillium olsonii,      the  Phoma  genus fungus is  Phoma multirostrata , and   the  Trichoderma  genus fungus is  Trichoderma asperellum.      
     
     
         7 . A tomato pathogenic fungus detecting method comprising selectively detecting a tomato pathogenic fungus using the detecting apparatus according to  claim 1 .