System, method and computer-accessible medium for multi-plane imaging of neural circuits

Abstract

An exemplary device can be provided which can include, for example, a radiation source(s) configured to generate a first radiation(s), a spatial light modulator (SLM) arrangement(s) configured to receive the first radiation(s) and generate a second radiation(s) based on the first radiation(s), and a galvanometer(s) configured to receive the second radiation(s), generate a third radiation(s) based on the second radiation(s), and provide the third radiation(s) to a sample(s).

1 . A device, comprising:
 at least one radiation source configured to generate at least one first radiation;   at least one spatial light modulator (SLM) arrangement configured to receive the at least one first radiation and generate at least one second radiation based on the at least one first radiation; and   at least one galvanometer configured to receive the at least one second radiation, generate at least one third radiation based on the at least one second radiation, and provide the at least one third radiation to at least one sample.   wherein the SLM arrangement includes at least one optical component configured to resize the at least one second radiation to at least substantially match a size of an acceptance aperture of the at least one galvanometer.   
     
     
         2 . The device of  claim 1 , wherein the at least one SLM arrangement includes:
 a SLM; and   a pre-SLM afocal telescope configured to resize the at least one first radiation to match an area of the SLM.   
     
     
         3 . The device of  claim 2 , wherein the at least one SLM arrangement further includes:
 a plurality of folding mirrors configured to redirect the at least one first radiation to the pre-SLM and   wherein the at least optical component includes a post SLM afocal telescope.   
     
     
         4 . The device of  claim 3 , wherein the at least one SLM arrangement further includes at least one broadband waveplate located between the pre-SLM afocal telescope and the SLM. 
     
     
         5 . The device of  claim 4 , wherein the at least one broadband waveplate is configured to rotate a polarization of the at least one first radiation to cause the at least one radiation to be parallel with an active axis of the SLM. 
     
     
         6 . The device of  claim 1 , wherein the at least one SLM arrangement is configured to split the at least one first radiation into the radiation beamlets which are the at least one second radiation. 
     
     
         7 . The device of  claim 6 , wherein the at least one SLM arrangement is further configured to independently dynamically control each of the radiation beamlets. 
     
     
         8 . The device of  claim 6 , wherein the at least one SLM arrangement splits the at least one first radiation into the radiation beamlets by imprinting a phase profile across the at least one first radiation. 
     
     
         9 . The device of  claim 6 , wherein the at least one galvanometer is further configured to direct each of the radiation beamlets to at least one of (i) a different area of the at least one sample, or (ii) a different plane of the at least one sample. 
     
     
         10 . (canceled) 
     
     
         11 . The device of  claim 1 , wherein the at least one radiation source is at least one laser source. 
     
     
         12 . The device of  claim 1 , further comprising at least one pocket cell located between the at least one radiation source and the at least one SLM arrangement. 
     
     
         13 . The device of  claim 12 , wherein the at least one pocket cell is configured to modulate an intensity of the at least one first radiation. 
     
     
         14 . The device of  claim 1 , further comprising a computer processing arrangement configured to generate at least one image of the at least one sample based on at least one fourth radiation received from the at least one sample that is based on the at least one third radiation. 
     
     
         15 . The device of  claim 1 , further comprising a computer processing arrangement configured to generate at least one image of the at least one sample based on a plurality of resultant radiations received from the at least one sample that are based on the radiation beamlets. 
     
     
         16 . The device of  claim 15 , wherein a first number of the resultant radiations is based on a second number of the radiation beamlets. 
     
     
         17 . The device of  claim 16 , wherein the second number of the radiation beamlets is based on a third number of the planes of the at least one sample. 
     
     
         18 . The device of  claim 17 , wherein the computer processing arrangement is further configured to generate a third number of images of the at least one sample based on the resultant radiations. 
     
     
         19 . The device of  claim 15 , wherein the at least one image includes a plurality of images. 
     
     
         20 . The device of  claim 19 , wherein the computer processing arrangement is further configured to generate at least one multiplane image based on the images. 
     
     
         21 . The device of  claim 20 , wherein the at least one multiplane image is generated by interleaving the images into the at least one multiplane image. 
     
     
         22 . The device of  claim 20 , wherein the computer processing arrangement is further configured to correct brain motion artifacts in the images based on a pyramid procedure. 
     
     
         23 . A method, comprising:
 generating at least one radiation;   providing the at least one radiation to at least one spatial light modulator (SLM) arrangement;   splitting the at least one radiation into a plurality of radiation beamlets using the at least one SLM arrangement;   resizing the radiation beamlets to at least substantially match a size of an acceptance aperture of at least one galvanometer; and   directing the radiation beamlets to at least one sample using the at least one galvanometer.   
     
     
         24 .- 39  (canceled)