US2021017565A1PendingUtilityA1

Process for manipulating the level of glycan content of a glycoprotein

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Assignee: AMGEN INCPriority: Dec 1, 2014Filed: Oct 2, 2020Published: Jan 21, 2021
Est. expiryDec 1, 2034(~8.3 yrs left)· nominal 20-yr term from priority
Inventors:Daniel R. LeiskeMichael T. Trentalange
C12P 21/02C12P 21/00C12N 2523/00C12N 2500/60C12N 2500/20C12N 5/0602C07K 16/00C07K 14/775C07K 14/52C07K 14/00C07K 16/242A61K 38/00C07K 2317/732C07K 16/241C07K 2317/41A61P 43/00C07K 2317/734C12P 21/005
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Claims

Abstract

The present invention provides a method for manipulating the fucosylated glycan content on a recombinant protein.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 .- 25 . (canceled) 
     
     
         26 . A serum free, chemically defined mammalian cell culture medium comprising from 10 to 100 ppb copper and from 50 to 1000 nM manganese. 
     
     
         27 . The cell culture medium according to  claim 26 , wherein the concentration of copper is 100 ppb. 
     
     
         28 . The cell culture medium according to  claim 26 , wherein the concentration of manganese is 1000 nM. 
     
     
         29 . The cell culture medium according to  claim 26 , wherein the pH is about 7.0.

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