Polypeptides Having Lysozyme Activity and Polynucleotides Encoding Same

Abstract

The present invention relates to isolated polypeptides having lysozyme activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

1 . An isolated polypeptide having lysozyme activity, selected from the group consisting of:
 (a) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 4 or to the mature polypeptide of SEQ ID NO: 8;   (b) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 3 or to the mature polypeptide coding sequence of SEQ ID NO: 7;   (c) a polypeptide encoded by a polynucleotide that hybridizes under medium-high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 3 or SEQ ID NO: 7, or the full-length complement thereof;   (d) a variant of the mature polypeptide of SEQ ID NO: 4 or SEQ ID NO: 8, comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions; and   (e) a fragment of the polypeptide of (a), (b), (c) or (d) that has lysozyme activity.   
     
     
         2 . The polypeptide of  claim 1 , selected among polypeptides comprising or consisting of SEQ ID NO: 4 or SEQ ID NO: 8 or the mature polypeptide of SEQ ID NO: 4 or SEQ ID NO: 8. 
     
     
         3 . The polypeptide of  claim 2 , wherein the mature polypeptide is selected among amino acids 20 to 227 of SEQ ID NO: 4 or amino acids 1 to 208 of SEQ ID NO: 8. 
     
     
         4 . The polypeptide of  claim 1 , which is a variant of the mature polypeptide of SEQ ID NO: 4 or SEQ ID NO: 8 comprising a substitution, deletion, and/or insertion at one or more (several) positions. 
     
     
         5 . A composition comprising the polypeptide of  claim 1 . 
     
     
         6 . The composition of  claim 5  being a detergent composition. 
     
     
         7 - 9 . (canceled) 
     
     
         10 . An animal feed composition comprising the polypeptide of 
     
     
         11 . The animal feed composition of  claim 10 , which further comprises one or more amylases; phytases; xylanases; galactanases; alpha-galactosidases; proteases, phospholipases, beta-glucanases, or any mixture thereof. 
     
     
         12 . An animal feed additive comprising
 at least one polypeptide of  claim 1 ; and   at least one fat-soluble vitamin, and/or   at least one water-soluble vitamin, and/or   at least one trace mineral.   
     
     
         13 . The animal feed additive of  claim 12 , which further comprises one or more amylases; phytases; xylanases; galactanases; alpha-galactosidases; proteases, phospholipases, beta-glucanases, or any mixture thereof. 
     
     
         14 . The composition of  claim 5  being a bacterial genomic DNA extraction composition. 
     
     
         15 . The bacterial genomic DNA extraction composition of  claim 14  wherein the composition additionally comprises of one or more further lysozymes. 
     
     
         16 - 21 . (canceled) 
     
     
         22 . A method of isolating DNA from bacteria, comprising:
 (a) treating the isolated bacteria with the polypeptide of  claim 1 ; and   (b) recovering the bacterial DNA.   
     
     
         23 . An isolated polynucleotide encoding the polypeptide of  claim 1 . 
     
     
         24 . A nucleic acid construct or expression vector comprising the polynucleotide of  claim 23  operably linked to one or more control sequences that direct the production of the polypeptide in an expression host. 
     
     
         25 . A recombinant host cell comprising the polynucleotide of  claim 23  operably linked to one or more control sequences that direct the production of the polypeptide. 
     
     
         26 . (canceled) 
     
     
         27 . A method of producing a polypeptide having lysozyme activity, comprising:
 (a) cultivating the host cell of  claim 25  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         28 - 29 . (canceled)