Pcl patch tissue regeneration scaffold and method for manufacturing same

Abstract

Disclosed is a method of preparing PCL patch tissue regeneration scaffold comprising preparing solution by adding polycaprolactone (PCL) and an acid to an organic solvent; preparing an electrospinning solution by adding a growth factor to the solution and stirring; and collecting nanofibers arranged in a spindle shape on a collector by connecting the electrospinning solution prepared to a syringe pump and operating the electrospinning device.

1 . A method of preparing PCL patch tissue regeneration scaffold comprising:
 preparing solution by adding polycaprolactone (PCL) and an acid to an organic solvent and stirring;   preparing an electrospinning solution by adding a growth factor to the solution and stirring; and   collecting nanofibers arranged in a spindle shape on a collector by connecting the electrospinning solution prepared to a syringe pump and operating the electrospinning device.   
     
     
         2 . (canceled) 
     
     
         3 . The method of preparing PCL patch tissue regeneration scaffold of  claim 1 , wherein a concentration of the polycaprolactone in the organic solvent is 70 to 90 mg/ml. 
     
     
         4 - 5 . (canceled) 
     
     
         6 . The method of preparing PCL patch tissue regeneration scaffold of  claim 1 , wherein the growth factor is any one selected from the group consisting of epithelial growth factor (EGF), fibroblast growth factor (FGF), insulin positive growth factor binding protein (IGFBP) and combination thereof. 
     
     
         7 . The method of preparing PCL patch tissue regeneration scaffold of  claim 1 , wherein in a step of preparing the electrospinning solution, the growth factor of 0.16 to 16 μg/ml is added to the solution. 
     
     
         8 . (canceled) 
     
     
         9 . The method of preparing PCL patch tissue regeneration scaffold of  claim 1 , wherein in a step of collecting the nanofibers, a electrospinning is operated by connecting the electrospinning solution prepared to a syringe pump and discharging at a rate of 0.2 to 3.0 μl/hr. 
     
     
         10 . (canceled) 
     
     
         11 . The method of preparing PCL patch tissue regeneration scaffold of  claim 1 , wherein the collector is in a cylindrical form for receiving needles therein. 
     
     
         12 . The method of preparing PCL patch tissue regeneration scaffold of  claim 1 , wherein in a step of preparing the solution, polycaprolactone and polyethylene glycol are added to the organic solvent. 
     
     
         13 . (canceled) 
     
     
         14 . The method of preparing PCL patch tissue regeneration scaffold of  claim 12 , wherein a concentration of the polycaprolactone in the organic solvent is 100 to 160 mg/ml. 
     
     
         15 . The method of preparing PCL patch tissue regeneration scaffold of  claim 12 , wherein a concentration of the polyethylene glycol in the organic solvent is 40 to 100 mg/ml. 
     
     
         16 . A PCL patch tissue regeneration scaffold comprising polycaprolactone as a nanofiber arranged in a spindle shape. 
     
     
         17 . The PCL patch tissue regeneration scaffold of  claim 16 , further comprising a growth factor. 
     
     
         18 . The PCL patch tissue regeneration scaffold of  claim 17 , which comprises polycaprolactone and a growth factor in a weight ratio of 10 4  to 10 6 :1. 
     
     
         19 - 25 . (canceled) 
     
     
         26 . A composition for preventing or treating chronic tympanic perforation, comprising a PCL patch tissue regeneration scaffold comprising polycaprolactone as a nanofiber arranged in a spindle shape. 
     
     
         27 . (canceled) 
     
     
         28 . A composition for preventing or treating chronic tympanic perforation of  claim 26 , comprising a PCL and PEG patch tissue regeneration scaffold comprising polyethylene glycol wherein in a polycaprolactone. 
     
     
         29 . A composition for preventing or treating chronic tympanic perforation of  claim 28 , wherein the PCL and PEG patch tissue regeneration scaffold further comprises growth factors. 
     
     
         30 . A composition for preventing or treating chronic tympanic perforation of  claim 29 , wherein the PCL and PEG patch tissue regeneration scaffold comprises polycaprolactone and a growth factor in weight ratio of (7×10 4 -7×10 6 ):1. 
     
     
         31 . A composition for preventing or treating chronic tympanic perforation of  claim 29 , wherein the PCL and PEG patch tissue regeneration scaffold comprises polyethylene glycol and a growth factor in weight ratio of (3×10 4 -3×10 6 ):1. 
     
     
         32 . A composition for preventing or treating chronic tympanic perforation of  claim 29 , wherein the PCL and PEG patch tissue regeneration scaffold comprises polycaprolactone and polyethylene glycol in weight ratio of (2-4):1.