Serine proteases

Abstract

The present disclosure relates to serine proteases and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.

1 - 23 . (canceled) 
     
     
         24 . A method of cleaning, comprising contacting a surface or an item in need of cleaning with a composition comprising a surfactant and a polypeptide or active fragment thereof of the BspAP02013-clade; and optionally further comprising the step of rinsing said surface or item after contacting said surface or item with said composition. 
     
     
         25 . The method of  claim 24 , wherein said item is dishware or fabric. 
     
     
         26 - 36 . (canceled) 
     
     
         37 . The method of  claim 24 , wherein the polypeptide or active fragment thereof comprises one or more motif selected from:
 (i) DTGIXXXHXDLXXXXXGGXSVFTDSXXXXXXXDXXGH (SEQ ID NO:11) motif, wherein the initial D is the active site Aspartic acid, the terminal H is the active site Histidine, and X is any amino acid;   (ii) DTGIXXXHXDLX a XXXXGGXSVFTDSXXXXXXXDXXGH (SEQ ID NO:12) motif, wherein the initial D is the active site Aspartic acid, the terminal H is the active site Histidine, X is any amino acid, and X a  is T, S or F or X a  is S or F;   (iii) DTGIXXXHXDLXXXXXGGXSVFTDSXXX b XXXXDXXGH (SEQ ID NO:13) motif, wherein the initial D is the active site Aspartic acid, the terminal H is the active site Histidine, X is any amino acid, and X b  is S or R or X b  is S;   (iv) DTGIXXXHXDLX a XXXXGGXSVFTDSXXX b XXXXDXXGH (SEQ ID NO:14) motif, wherein the initial D is the active site Aspartic acid; the terminal H is the active site Histidine; X is any amino acid; X a  is T, S or F or X a  is S or F; and X b  is S or R or X b  is S;   (v) DTGIXXXHXDLXANVXGGXSVFTDSANXDPFXDXXGH (SEQ ID NO:15) motif, wherein the initial D is the active site Aspartic acid and the terminal H is the active site Histidine, and X is any amino acid;   (vi) HXDLXANVXGGXS (SEQ ID NO:16) motif, wherein the initial D is the active site Aspartic acid, the terminal GGXS is in the outermost strand of the central beta sheet, and X is any amino acid;   (vii) GGXSVFTDSANXDPFXD (SEQ ID NO:17) motif, wherein the initial GGXS is in the outermost strand of the central beta sheet and X is any amino acid;   (viii) HXDLX a ANVXGGXS (SEQ ID NO:18) motif, wherein the initial D is the active site Aspartic acid; the terminal GGXS is in the outermost strand of the central beta sheet;   X a  is T, S or F or X a  is S or F; and X is any amino acid;   (ix) GGXSVFTDSANX b DPFXD (SEQ ID NO:19) motif, wherein the initial GGXS is in the outermost strand of the central beta sheet, X is any amino acid, and X b  is S or R or X b  is 5;   (x) DTGIXXXHXDLXXXXXGGXSVFXDXXXXXXXXDXXGH (SEQ ID NO:22) motif, wherein the initial D is the active site Aspartic acid and the terminal H is the active site Histidine, and X is any amino acid;   (xi) DTGIXXXHXDLXXNVXGGXSVFXDXXNXDPXXDXXGH (SEQ ID NO:23) motif, wherein the initial D is the active site Aspartic acid and the terminal H is the active site Histidine, and X is any amino acid; and   (x) an amino acid sequence having at least 80% amino acid sequence identity to an amino acid sequence of SEQ ID NO:3, 6, or 9.   
     
     
         38 . The method of  claim 37 , wherein the polypeptide or active fragment thereof does not comprise WP_012957833, ERN55058, WP_022626565, or WP_026690432, or optionally WP_047973355. 
     
     
         39 . The method of  claim 24 , wherein the surfactant is selected from the group consisting of an anionic surfactant, a cationic surfactant, a zwitterionic surfactant, an ampholytic surfactant, a semi-polar non-ionic surfactant, and a combination thereof. 
     
     
         40 . The method of  claim 24 , wherein the composition is a detergent composition. 
     
     
         41 . The composition of  claim 40 , wherein the detergent composition is selected from the group consisting of a laundry detergent, a fabric softening detergent, a dishwashing detergent, and a hard-surface cleaning detergent. 
     
     
         42 . The method of  claim 24 , wherein said composition further comprises at least one calcium ion and/or zinc ion; at least one stabilizer; from about 0.001% to about 1.0 weight % of said subtilisin or recombinant polypeptide; at least one bleaching agent; at least one adjunct ingredient; and/or one or more additional enzymes or enzyme derivatives selected from the group consisting of acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, xylosidases, metalloproteases, additional serine proteases, and combinations thereof. 
     
     
         43 . The method of  claim 24 , wherein said composition contains phosphate or is phosphate-free and/or contains borate or is borate-free. 
     
     
         44 . The method of  claim 24 , wherein said composition is a granular, powder, solid, bar, liquid, tablet, gel, paste or unit dose composition. 
     
     
         45 . The method of  claim 24 , wherein said composition is formulated at a pH of from about 8 to about 12.