Soybean plant and seed corresponding to transgenic event mon87712 and methods for detection thereof

Abstract

The present invention provides a transgenic soybean comprising event MON87712 that exhibits increased yield. The invention also provides cells, plant parts, seeds, plants, commodity products related to the event, and DNA molecules that are unique to the event and were created by the insertion of transgenic DNA into the genome of a soybean plant. The invention further provides methods for detecting the presence of said soybean event nucleotide sequences in a sample, probes and primers for use in detecting nucleotide sequences that are diagnostic for the presence of said soybean event.

1 .- 26 . (canceled) 
     
     
         27 . A recombinant DNA molecule comprising the nucleotide sequence of SEQ ID NO:1 and SEQ ID NO:2 and a sequence encoding a zinc finger protein (BBX32). 
     
     
         28 . The recombinant DNA molecule of  claim 27 , further defined as comprising a nucleotide sequence of at least 55 consecutive nucleotides of SEQ ID NO:7 and at least 55 consecutive nucleotides of SEQ ID NO:8. 
     
     
         29 . The recombinant DNA molecule of  claim 27 , further defined as comprising the nucleotide sequence of SEQ ID NO:7 and SEQ ID NO:8. 
     
     
         30 . The recombinant DNA molecule of  claim 27 , further defined as comprising the nucleotide sequence of SEQ ID NO:3 and SEQ ID NO:4. 
     
     
         31 . A method of producing soybean seed, the method comprising crossing a soybean plant comprising a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:1-4, 7, 8, and complements thereof with itself or a different soybean plant to produce said soybean seed. 
     
     
         32 . A soybean seed produced by the method of  claim 31 , wherein the soybean seed comprises said nucleic acid molecule. 
     
     
         33 . The method of  claim 31 , further comprising crossing a plant grown from said soybean seed with a second soybean plant that does not comprise said nucleic acid molecule to produce a progeny plant of a subsequent generation. 
     
     
         34 . The method of  claim 33 , wherein the second soybean plant comprises a transgene conferring a desirable trait. 
     
     
         35 . The method of  claim 34 , wherein the transgene confers insect resistance, increased water use efficiency, increased drought tolerance, increased yield performance, increased nitrogen use efficiency, tolerance to nitrogen stress, increased seed quality, disease resistance, improved nutritional quality, or herbicide tolerance. 
     
     
         36 . A soybean seed produced by the method of  claim 35 , wherein the soybean seed comprises said nucleic acid molecule. 
     
     
         37 . The soybean seed of  claim 36 , wherein the soybean seed is a hybrid seed. 
     
     
         38 . The method of  claim 33 , further comprising:
 (a) crossing the progeny plant of the subsequent generation with itself to produce a progeny plant of a further subsequent generation;   (b) crossing the progeny plant of the further subsequent generation with itself; and   (c) repeating step (b) a sufficient number of times to generate an inbred plant.   
     
     
         39 . A soybean seed produced by the method of  claim 38 , wherein the soybean seed comprises said nucleic acid molecule. 
     
     
         40 . The soybean seed of  claim 39 , wherein the soybean seed is an inbred seed. 
     
     
         41 . A method of producing a soybean plant with increased yield, the method comprising:
 (a) crossing a soybean plant comprising a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:1-4, 7, 8, and complements thereof with itself or a different soybean plant, thereby producing seed;   (b) growing said seed to produce a plurality of progeny plants; and   (c) selecting a progeny plant with increased yield.