Processed adipose tissue

Abstract

The present disclosure provides tissue products produced from adipose-containing tissues, as well as methods for producing such tissue products. The tissue products can comprise decellularized and partially de-fatted tissues. In addition, the present disclosure provides systems and methods for using such products.

What is claimed is: 
     
         1 . A method of producing a tissue product, comprising:
 providing a sheet of tissue comprising adipose-containing transitional dermis;   treating the sheet of tissue to remove substantially all cellular material from the tissue; and   processing the sheet of tissue to partially remove lipid components such that the tissue product retains between about 30% and 50% lipid content as a percentage of the overall tissue product by mass following processing.   
     
     
         2 . The method of  claim 1 , wherein substantially all cellular material is removed using one or more detergents. 
     
     
         3 . The method of  claim 2 , wherein the one or more detergents comprise at least a polyethylene glycol, Tris[2-(dimethylamino)ethyl]amine, sodium dodecyl sulfate, sodium deoxycholate, and polyoxyethylene (20) sorbitan mono-oleate. 
     
     
         4 . The method of  claim 1 , wherein processing the sheet of tissue to partially remove lipid components comprises exposing the sheet of tissue to an elevated temperature of about 40-50° C., to ultrasonic energy, or a combination of the elevated temperature and the ultrasonic energy. 
     
     
         5 . The method of  claim 4 , wherein the adipose-containing tissue is exposed to ultrasonic energy of about 20 to 2000 watts per square meter at a frequency between about 20 to 400 kilohertz. 
     
     
         6 . The method of  claim 1 , wherein the tissue product comprises at least about 3% extracellular matrix components as a percentage of the overall tissue product by mass following processing. 
     
     
         7 . The method of  claim 1 , further comprising exposing the tissue product to E-beam radiation. 
     
     
         8 . The method of  claim 1 , further comprising storing the tissue product in an aqueous solution or drying the tissue product for storage. 
     
     
         9 . The method of  claim 1 , wherein the drying includes freeze-drying. 
     
     
         10 . A method of treatment comprising:
 selecting a tissue site in a patient; and   implanting a tissue product comprising a sheet of decellularized adipose-containing transitional dermal matrix, wherein a portion of lipid components have been removed from the sheet of decellularized adipose-containing transitional dermal matrix such that the tissue product retains between about 30% and 50% lipid content as a percentage of the overall tissue product by mass.   
     
     
         11 . The method of  claim 10 , wherein the tissue product has sufficient lipid content to provide a soft and malleable material capable of molding or deforming to fill the tissue site while having a reduced lipid content sufficient to avoid significant inflammation following implantation. 
     
     
         12 . The method of  claim 10 , wherein the tissue product comprises sufficient extracellular matrix components to provide structural support for the lipid components of the tissue product. 
     
     
         13 . The method of  claim 12 , wherein the tissue product has at least about 3% extracellular matrix components as a percentage of the overall tissue product by mass. 
     
     
         14 . The method of  claim 10 , wherein the implanted tissue product promotes native adipose deposition, native cell migration, native cell proliferation, or revascularization in or near the implanted tissue product, while avoiding significant inflammation in or near the implanted tissue product. 
     
     
         15 . The method of  claim 10 , wherein the tissue product does not harden significantly following implantation. 
     
     
         16 . The method of  claim 10 , wherein the tissue site comprises adipose tissue or soft tissue. 
     
     
         17 . The method of  claim 10 , wherein the tissue product is implanted following surgical removal of bulk tissue from a patient. 
     
     
         18 . The method of  claim 17 , wherein the tissue site comprises a breast. 
     
     
         19 . The method of  claim 10 , wherein the tissue product is implanted to augment a tissue site. 
     
     
         20 . The method of  claim 10 , wherein the tissue site is a breast, a face, a buttock, an abdomen, a hip, or a thigh.