Method for assisting detection of alzheimer's disease or mild cognitive impairment

Abstract

Disclosed is a method for assisting the detection of Alzheimer's disease or mild cognitive impairment, the method for assisting the highly accurate detection of Alzheimer's disease or mild cognitive impairment. In the method for assisting the detection of Alzheimer's disease or mild cognitive impairment, the amount of at least one miRNA selected from miR-122, miR-144, let-7f, miR-128-3p and miR-107 contained in a test sample separated from a living body is used as an indicator. A larger amount of miR-122 than that in a healthy individual, or a smaller amount of at least one miRNA selected from miR-144, let-7f, miR-128-3p and miR-107 than that in a healthy individual is indicative that the living body is more likely to have developed Alzheimer's disease or mild cognitive impairment.

1 . A method of detecting and treating Alzheimer's disease or mild cognitive impairment in a subject, the method comprising the step of:
 measuring an amount of each of miR-122 and at least one microRNA selected from let-7f and miR-144 present in blood collected from the subject;   comparing the measured amount of each of miR-122 and the selected microRNA in the subject with an amount of each of miR-122 and the selected microRNA in a healthy individual; and   administering a medicine to slow down the progression of the Alzheimer's disease or mild cognitive impairment to the subject whose amount of miR-122 in the blood is larger than that in the healthy individual and whose amount of the selected microRNA in the blood is smaller than that in the healthy individual.   
     
     
         2 . The method according to  claim 1 , wherein the at least one microRNA is let-7f. 
     
     
         3 . The method according to  claim 1 , wherein the at least one microRNA is miR-144. 
     
     
         4 . The method according to  claim 1 , wherein said measuring is performed by quantitative real-time PCR or a next generation sequencer. 
     
     
         5 . The method according to  claim 1 , wherein an amount of let-7g-5p present in the blood is also measured, and the measured amount of let-7g-5p is used as an internal control to normalize the measured amount of each of miR-122 and the selected microRNA.