Milk-based protein hydrolysates and infant formulae and nutritional compositions made thereof

Abstract

A composition including protein hydrolysates derived from milk is obtained by the treatment of enzymes derived from microorganisms. A trypsin-like enzyme and a chymotrypsin-like enzyme are used. The composition is in particular intended for inducing tolerance in infants with the effect of modulating the potential occurrence of allergies later in life. The composition can also be used in sick adult patients. Preferably the composition is an infant formula, infant follow-up formula, growing-up milk or baby food or an enteral complete nutritional composition.

The invention is claimed as follows: 
     
         1 . A composition comprising milk-based protein hydrolysate obtainable by treatment of a solution of a milk-based proteinaceous material with:
 a) at least one trypsin-like endopeptidase produced by fermentation of a cell of an organism of a strain of  Fusarium oxysporum  having at least 75% sequence identity to the sequence SWISSPROT No. P35049, and   b) at least one chymotrypsin-like endopeptidase produced by fermentation of a cell of an organism of a strain of  Nocardiopsis  Sp and having at least 75% sequence identity to the sequence EMBL CDS CAI94179,   wherein the ratio of the trypsin-like endopeptidase to the chymotrypsin-like endopeptidase based on weight of enzyme is 5:1 to 20:1.   
     
     
         2 . The composition of  claim 1 , wherein the at least one trypsin-like endopeptidase has at least 80% sequence identity to the sequence SWISSPROT No. P35049. 
     
     
         3 . The composition of  claim 1 , wherein the at least one trypsin-like endopeptidase has at least 85% sequence identity to the sequence SWISSPROT No. P35049. 
     
     
         4 . The composition of  claim 1 , wherein the at least one trypsin-like endopeptidase has at least 90% sequence identity to the sequence SWISSPROT No. P35049. 
     
     
         5 . The composition of  claim 1 , wherein the at least one trypsin-like endopeptidase has at least 95% sequence identity to the sequence SWISSPROT No. P35049. 
     
     
         6 . The composition of  claim 1 , wherein the at least one chymotrypsin-like endopeptidase has at least 80% sequence identity to the sequence EMBL CDS CAI94179. 
     
     
         7 . The composition of  claim 1 , wherein the at least one chymotrypsin-like endopeptidase has at least 85% sequence identity to the sequence EMBL CDS CAI94179. 
     
     
         8 . The composition of  claim 1 , wherein the at least one chymotrypsin-like endopeptidase has at least 90% sequence identity to the sequence EMBL CDS CAI94179. 
     
     
         9 . The composition of  claim 1 , wherein the at least one chymotrypsin-like endopeptidase has at least 95% sequence identity to the sequence EMBL CDS CAI94179. 
     
     
         10 . A method comprising obtaining milk-based protein hydrolysate by treatment of a solution of a milk-based proteinaceous material with:
 a) at least one trypsin-like endopeptidase produced by fermentation of a cell of an organism of a strain of  Fusarium oxysporum  having at least 75% sequence identity to the sequence SWISSPROT No. P35049, and   b) at least one chymotrypsin-like endopeptidase produced by fermentation of a cell of an organism of a strain of  Nocardiopsis  Sp and having at least 75% sequence identity to the sequence EMBL CDS CAI94179,   wherein the ratio of the trypsin-like endopeptidase to the chymotrypsin-like endopeptidase based on weight of enzyme is 5:1 to 20:1.   
     
     
         11 . The method of  claim 10 , wherein the at least one trypsin-like endopeptidase has at least 80% sequence identity to the sequence SWISSPROT No. P35049. 
     
     
         12 . The method of  claim 10 , wherein the at least one trypsin-like endopeptidase has at least 85% sequence identity to the sequence SWISSPROT No. P35049. 
     
     
         13 . The method of  claim 10 , wherein the at least one trypsin-like endopeptidase has at least 90% sequence identity to the sequence SWISSPROT No. P35049. 
     
     
         14 . The method of  claim 10 , wherein the at least one trypsin-like endopeptidase has at least 95% sequence identity to the sequence SWISSPROT No. P35049. 
     
     
         15 . The method of  claim 10 , wherein the at least one chymotrypsin-like endopeptidase has at least 80% sequence identity to the sequence EMBL CDS CAI94179. 
     
     
         16 . The method of  claim 10 , wherein the at least one chymotrypsin-like endopeptidase has at least 85% sequence identity to the sequence EMBL CDS CAI94179. 
     
     
         17 . The method of  claim 10 , wherein the at least one chymotrypsin-like endopeptidase has at least 90% sequence identity to the sequence EMBL CDS CAI94179. 
     
     
         18 . The method of  claim 10 , wherein the at least one chymotrypsin-like endopeptidase has at least 95% sequence identity to the sequence EMBL CDS CAI94179.