Xylanases for solubilising arabinoxylan-containing material

Abstract

The present invention relates to a method for solubilising arabinoxylan-containing material (particularly insoluble arabinoxylan-containing material), comprising admixing a xylan-containing material with a xylanase comprising a polypeptide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 1, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11 or SEQ ID No. 15, or a variant, homologue, fragment or derivative thereof having at least 75% identity with SEQ ID No. 3 or SEQ ID No. 2 or SEQ ID No. 1 or SEQ ID No. 9 or SEQ ID No. 10 or SEQ ID No. 11 or SEQ ID No. 15; or a polypeptide sequence which comprises SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 1, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11 or SEQ ID No. 15 with a conservative substitution of at least one of the amino acids; or a xylanase which is encoded by a nucleotide sequence shown herein as SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No.17 or SEQ ID No. 18, or a nucleotide sequence which can hybridize to SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No.17 or SEQ ID No. 18 under high stringency conditions, or a nucleotide sequence which has at least 75% identity with SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No.17 or SEQ ID No. 18, or a nucleotide sequence which differs from SEQ ID No. 6 or SEQ ID No. 5 or SEQ ID No. 4 or SEQ ID No. 12 or SEQ ID No. 13 or SEQ ID No. 14 or SEQ ID No. 16 or SEQ ID No.17 or SEQ ID No. 18 due to the degeneracy of the genetic code, or a xylanase obtainable (or obtained) from Fusarium verticilloides . The present invention also relates to a novel xylanase comprising (or consisting of) a polypeptide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1, or a variant, homologue, fragment or derivative thereof having at least 99% identity with SEQ ID No. 3 or SEQ ID No. 2 or SEQ ID No. 1; or a xylanase which is encoded by a nucleotide sequence shown herein as SEQ ID No. 6, SEQ ID No. 5 or SEQ ID No. 4, or a nucleotide sequence which can hybridize to SEQ ID No. 4 or SEQ ID No. 5 under high stringency conditions, or a nucleotide sequence which has at least 97.7% identity (preferably 98% identity) with SEQ ID No. 6, SEQ ID No. 5 or SEQ ID No. 4. The present invention yet further relates to methods relating to feedstuffs, malting and brewing, processing of grain-based materials such as during the production of bioethanol or biochemical (e.g. bio-based isopropanol), or wheat gluten-starch separation processes and the like.

1 - 17 . (canceled) 
     
     
         18 . A recombinant host cell comprising a nucleic acid which encodes a polypeptide sharing 85% sequence identity to the amino acid sequence of SEQ ID NO:3. 
     
     
         19 - 26 . (canceled) 
     
     
         27 . The host cell of  claim 18 , wherein the host cell is a bacterial, fungal, or yeast cell. 
     
     
         28 . The host cell of  claim 27 , wherein the bacterial cell is a  Streptomyces  spp. or a  Bacillus  spp. 
     
     
         29 . The host cell of  claim 27 , wherein the fungal cell is a  Fusarium  spp., an  Aspergillus  spp., a  Thermomyces  spp., an  Acremonium  spp., a  Penicillium  spp., a  Mucor  spp., a  Neurospora  spp., or a  Trichoderma  spp. 
     
     
         30 . A method for degrading for degrading arabinoxylan-containing material in a xylan-containing material comprising contacting the material with the polypeptide produced by the host cell of  claim 18 . 
     
     
         31 . The method of  claim 30 , wherein the xylan-containing material is selected from one or more of the group consisting of: a feed or feedstuff, a feed component, a grain-based material, a mash, a wort, a malt, malted barely, an adjunct, a barley mash, and a cereal flour. 
     
     
         32 . The method of  claim 31 , wherein the feed or feedstuff or feed component comprises or consists of corn, Distillers Dried Grains with Solubles (DDGS), corn Distillers Dried Grain Solubles (cDDGS), wheat, wheat bran or a combination thereof. 
     
     
         33 . The method according to  claim 31  wherein the feed or feedstuff is a corn-based feedstuff. 
     
     
         34 . The method of  claim 30 , wherein the polypeptide is used in combination with one or more of the enzymes selected from the group consisting of a protease, an amylase, and a phytase.