Methods, Systems, And Compositions For Determining Blood Clot Formation, And Uses Thereof

Abstract

A method is directed to determining a thrombosis function and includes flowing a fluid sample over a surface having a fixed endothelial cell monolayer. The method further includes stimulating the fixed endothelial cell monolayer to induce formation of a clot, the clot being formed via interaction between the fixed endothelial cell monolayer and the fluid sample. In response to the clot formation, the method further includes determining a thrombosis function associated with the fluid sample and the fixed endothelial cell monolayer.

1 - 65 . (canceled) 
     
     
         66 . A method of making microfluidic devices, comprising:
 a) providing a microfluidic device comprising a channel;   b) culturing endothelial cells in the channel so as to form a lumen;   c) fixing the endothelial cells; and   d) shipping the device.   
     
     
         67 . The method of  claim 66 , wherein the fixing is done with a fixative agent selected from the group consisting of formaldehyde, paraformaldehyde, formalin, glutaraldehyde, mercuric chloride-based fixatives, Helly's solution, Zeneker's solution, precipitating fixatives, ethanol, methanol, acetone, dimethyl suberimidate, Bouin's fixative, a decellularization solvent, a detergent, a high pH solution, and a combination of two or more thereof. 
     
     
         68 . The method of  claim 66 , wherein the method further comprises the step of storing the microfluidic device after step c). 
     
     
         69 . The method of  claim 68 , wherein the microfluidic device is stored at a temperature between 4° C. and 10° C. 
     
     
         70 . The method of  claim 68 , wherein the microfluidic device is stored at a temperature of about 4° C. or lower. 
     
     
         71 . The method of  claim 68 , wherein the microfluidic device is stored at room temperature. 
     
     
         72 . The method of  claim 68 , wherein the microfluidic device is stored for 1 day or longer. 
     
     
         73 . The method of  claim 68 , wherein the microfluidic device is stored for 5 days or longer. 
     
     
         74 . A method of making microfluidic devices, comprising:
 a) providing a microfluidic device comprising a channel;   b) culturing endothelial cells in the channel of each microfluidic device so as to form a lumen;   c) fixing the endothelial cells; and   d) storing the plurality of microfluidic devices.   
     
     
         75 . The method of  claim 74 , wherein the fixing is done with a fixative agent selected from the group consisting of formaldehyde, paraformaldehyde, formalin, glutaraldehyde, mercuric chloride-based fixatives, Helly's solution, Zeneker's solution, precipitating fixatives, ethanol, methanol, acetone, dimethyl suberimidate, Bouin's fixative, a decellularization solvent, a detergent, a high pH solution, and a combination of two or more thereof. 
     
     
         76 . The method of  claim 74 , wherein the microfluidic device is stored at a temperature between 4° C. and 10° C. 
     
     
         77 . The method of  claim 74 , wherein the microfluidic device is stored at a temperature of about 4° C. or lower. 
     
     
         78 . The method of  claim 74 , wherein the microfluidic device is stored at room temperature. 
     
     
         79 . The method of  claim 74 , wherein the microfluidic device is stored for 1 day or longer. 
     
     
         80 . The method of  claim 74 , wherein the microfluidic device is stored for 5 days or longer.