US2020291380A1PendingUtilityA1
Tethering cysteine residues using cyclic disulfides
Est. expiryAug 18, 2035(~9.1 yrs left)· nominal 20-yr term from priority
C12Y 203/02013C12N 9/1044A61K 38/00C12N 9/96A61K 31/385A61K 31/095C07K 2319/00C12Y 115/01001C12N 9/80C07K 19/00C12N 9/0089
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Claims
Abstract
Described herein are compounds and methods for tethering proteins. For example, dimers of Protein X listed in Table 1 are described, where the dimers are formed by the covalent bonding of a cysteine on the first monomer to a cysteine on the second monomer via a cyclic disulfide linker. The covalently attached dimers exhibit increased stabilization and can be used to treat neurodegenerative diseases (such as, for example, Parkinson's Disease, ALS, Alzheimer's Disease, Huntington's Disease, Epilepsy, Frontotemporal Dementia, and/or DMD), cancer, autoimmune disease, and/or Celiac disease.
Claims
exact text as granted — not AI-modified1 . A stabilized analogue comprising a first Protein X and a second Protein X cross-linked to one another by a compound of Formula I or a compound of Formula II, wherein
the first Protein X comprises a first cysteine residue and is listed in Table 1; the second Protein X comprises a second cysteine residue and is listed in Table 1; the compound of Formula I is
wherein
Y is S, S═O, or S(═O) 2 ;
n is 0, 1, 2, 3, or 4; and
R is independently selected from the group consisting of —H, —OH, —NH 2 , —NHR′, —N(R′) 2 , alkyl, —OMs, —OTs, —OTf, and —CO 2 H; or any two geminal R groups, taken together, form an imine; or any two vicinal R groups, taken together, form a ring; wherein any alkyl or imine may be substituted with a carbamide, a carboxylate, or a hydroxyl; and
R′ is alkyl or aryl; and
the compound of Formula II is
wherein
R″ is —H, alkyl, or aryl, or both R″, taken together, form a ring; wherein any alkyl, aryl, or ring may be substituted with —OH, alkyl, or halo.
2 . The stabilized analogue of claim 1 , wherein the first Protein X and the second Protein X have at least 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence homology or identity to any one of the Protein X listed in Table 1.
3 . The stabilized analogue of claim 1 wherein the first Protein X and the second Protein X are derived from different Protein X shown in Table 1.
4 . The stabilized analogue of claim 1 , wherein the first Protein X and the second Protein X are derived from the same Protein X shown in Table 1.
5 . The stabilized analogue of claim 4 , wherein the first Protein X and the second Protein X are at least 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% homologous or identical to one another.
6 . The stabilized analogue of claim 1 , wherein the compound is a compound of Formula I; and the compound is selected from the group consisting of
7 . The stabilized analogue of claim 1 , wherein the compound is a compound of Formula II, and the compound is
8 . The stabilized analogue of claim 1 , wherein the stabilized analogue is a compound of Formula III or Formula IV:
wherein
Y is S, S═O, or S(═O) 2 ;
n is 0, 1, 2, 3, or 4;
R is independently selected from the group consisting of —H, —OH, —NH 2 , —NHR′, —N(R′) 2 , alkyl, —OMs, —OTs, —OTf, and —CO 2 H; or any two geminal R groups, taken together, form an imine; or any two vicinal R groups, taken together, form a ring; wherein any alkyl or imine may be substituted with a carbamide, a carboxylate, or a hydroxyl; and
R′ is alkyl or aryl; and
R″ is —H, alkyl, or aryl, or both R″, taken together, form a ring; wherein any alkyl, aryl, or ring may be substituted with —OH, alkyl, or halo.
9 . The stabilized analogue of claim 1 , wherein the stabilized analogue has reduced activity as compared to Protein X.
10 . The stabilized analogue of claim 1 , wherein the stabilized analogue has increased activity as compared to Protein X.
11 . A method of treating or preventing Disease Y, comprising administering to a subject in need thereof a therapeutically effective amount of the stabilized analogue of claim 1 , wherein Disease Y is a neurodegenerative disease (such as, for example, Parkinson's Disease, ALS, Alzheimer's Disease, Huntington's Disease, Epilepsy, Frontotemporal Dementia, and/or DMD), cancer, autoimmune disease, Celiac disease, and/or any of the Disease Y listed in Table 1.
12 . A method of treating or preventing Disease Y, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of Formula I or a compound of Formula II, wherein the compound of Formula I is
wherein
Y is S, S═O, or S(═O) 2 ;
n is 0, 1, 2, 3, or 4; and
R is independently selected from the group consisting of —H, —OH, —NH 2 , —NHR′, —N(R′) 2 , alkyl, —OMs, —OTs, —OTf, and —CO 2 H; or any two geminal R groups, taken together, form an imine; or any two vicinal R groups, taken together, form a ring; wherein any alkyl or imine may be substituted with a carbamide, a carboxylate, or a hydroxyl; and
R′ is alkyl or aryl; and
the compound of Formula II is
wherein
R″ is —H, alkyl, or aryl, or both R″, taken together, form a ring; wherein any alkyl, aryl, or ring may be substituted with —OH, alkyl, or halo, wherein Disease Y is a neurodegenerative disease (such as, for example, Parkinson's Disease, ALS, Alzheimer's Disease, Huntington's Disease, Epilepsy, Frontotemporal Dementia, and/or DMD), cancer, autoimmune disease, Celiac disease, and/or any of the Disease Y listed in Table 1.
13 . The method of claim 12 , wherein the compound is a compound of Formula I, and the compound is selected from:
14 . The method of claim 12 , wherein the compound is a compound of Formula II, and the compound is
15 . The method of claim 12 , wherein the compound of Formula I or the compound of Formula II forms the stabilized analogue of Formula III or Formula IV.
16 . A method comprising the step of contacting a compound of Formula I or a compound of Formula II with a first Protein X and a second Protein X under conditions suitable for cross-linking the first Protein X to the second Protein X, thereby cross-linking the first Protein X to the second Protein X, wherein
the first Protein X comprises a first cysteine residue and is listed in Table 1; the second Protein X comprises a second cysteine residue and is listed in Table 1; the compound of Formula I is
wherein
Y is S, S═O, or S(═O) 2 ;
n is 0, 1, 2, 3, or 4; and
R is independently selected from the group consisting of —H, —OH, —NH 2 , —NHR′, —N(R′) 2 , alkyl, —OMs, —OTs, —OTf, and —CO 2 H; or any two geminal R groups, taken together, form an imine; or any two vicinal R groups, taken together, form a ring; wherein any alkyl or imine may be substituted with a carbamide, a carboxylate, or a hydroxyl; and
R′ is alkyl or aryl; and
the compound of Formula II is
wherein
R″ is —H, alkyl, or aryl, or both R″, taken together, form a ring; wherein any alkyl, aryl, or ring may be substituted with —OH, alkyl, or halo.
17 . The method of claim 16 , wherein the first Protein X and the second Protein X have at least 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence homology or identity to any one of the Protein X listed in Table 1; and the compound is a compound of Formula I
wherein
Y is S, S═O, or S(═O) 2 ;
n is 0, 1, 2, 3, or 4; and
R is independently selected from the group consisting of —H, —OH, —NH 2 , —NHR′, —N(R′) 2 , alkyl, —OMs, —OTs, —OTf, and —CO 2 H; or any two geminal R groups, taken together, form an imine; or any two vicinal R groups, taken together, form a ring; wherein any alkyl or imine may be substituted with a carbamide, a carboxylate, or a hydroxyl; and
R′ is alkyl or aryl.
18 . The method of claim 16 , wherein the compound is a compound of Formula I; and the compound is selected from:
19 . The method of claim 16 , wherein the first Protein X and the second Protein X have at least 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence homology or identity to any one of the Protein X listed in Table 1; and the compound is a compound of Formula II
wherein
R″ is —H, alkyl, or aryl, or both R″, taken together, form a ring; wherein any alkyl, aryl, or ring may be substituted with —OH, alkyl, or halo.
20 . The method of claim 16 , wherein the compound is a compound of Formula II, and the compound is
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