Metal/radiometal-labeled psma inhibitors for psma-targeted imaging and radiotherapy

Abstract

Low-molecular weight gadolinium (Gd)-based MR contrast agents for PSMA-specific T 1 -weighted MR imaging are disclosed. The (Gd)-based MR contrast agents exhibit high binding affinity for PSMA and exhibit specific T 1 contrast enhancement at PSMA+ cells. The PSMA-targeted Gd-based MR contrast agents can be used for PSMA-targeted imaging in vivo. 86 Y-labeled PSMA-binding ureas also are provided, wherein the PSMA-binding ureas also are suitable for use with other radiotherapeutics.

That which is claimed: 
     
         1 . A compound of formula (I): 
       
         
           
           
               
               
           
         
         Z is tetrazole or CO 2 Q; 
         Q is H or a protecting group; 
         λ 1  and λ 2  are each independently NH or O; 
         a is an integer selected from the group consisting of 1, 2, 3 and 4; 
         c is an integer selected from the group consisting of 0, 1, 2, 3 and 4; 
         each R 1 , R 2  and R 4  is independently H or C 1 -C 4  alkyl; 
         each R 3  is independently H, C 1 -C 6  alkyl or C 2 -C 12  aryl; 
         W is independently O or S; 
         Y is —NH— and can be present or absent; 
         L is a linker selected from the group consisting of: 
       
       
         
           
           
               
               
           
         
       
       wherein:
 m is an integer selected from the group consisting of 1, 2, 3, 4, 5, 6, 7 and 8; 
 each R 5  is independently H or —COOR 6  wherein each R 6  is independently H or a C 1 -C 6  alkyl; 
 n is an integer selected from the group consisting of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12; 
 p is an integer selected from the group consisting of 1, 2, 3, 4, 5, 6, 7 and 8; 
 Ch is a chelating moiety that can comprise one or more metals or radiometals; or 
 a pharmaceutically acceptable salt thereof. 
 
     
     
         2 . The compound of  claim 1  wherein the chelating moiety is selected from the group consisting of: 
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         wherein q is an integer selected from the group consisting of 1, 2, 3, 4, 5, 6, 7, and 8. 
       
     
     
         3 . The compound of Formula (I), wherein the compound is selected from the group consisting of: 
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         wherein:
 M is a metal or a radiometal; or 
 a pharmaceutically acceptable salt thereof. 
 
       
     
     
         4 . The compound of  claim 1  wherein the metal is selected from the group consisting of Gd, Lu, Ac, Bi, Pb, Cu, In, Sc, and Y. 
     
     
         5 . The compound of  claim 1 , wherein the nonradioactive metal is Gd-157 (stable isotope). 
     
     
         6 . The compound of  claim 1 , wherein the radiometal is selected from the group consisting of Lu-177, Ac-225, Bi-213, Bi-212, Pb-212, Cu-67, In-111, Sc-47, and Y-90. 
     
     
         7 . The compound of  claim 1 , wherein the radiometal is selected from the group consisting of Y-86 and Sc-44. 
     
     
         8 . The compound of  claim 1 , wherein the radiometal is selected from the group consisting of Lu-177 and In-111. 
     
     
         9 . A method for imaging or treating one or more prostate-specific membrane antigen (PSMA) tumors or cells, the method comprising contacting the one or more tumors or cells with an effective amount of a compound of formula (I) and making an image, the compound of formula (I) comprising: 
       
         
           
           
               
               
           
         
         Z is tetrazole or CO 2 Q; 
         Q is H or a protecting group; 
         λ 1  and λ 2  are each independently NH or O; 
         a is an integer selected from the group consisting of 1, 2, 3 and 4; 
         c is an integer selected from the group consisting of 0, 1, 2, 3 and 4; 
         each R 1 , R 2  and R 4  is independently H or C 1 -C 4  alkyl; 
         each R 3  is independently H, C 1 -C 6  alkyl or C 2 -C 12  aryl; 
         W is independently O or S; 
         Y is —NH— and can be present or absent; 
         L is a linker, wherein the linker is selected from the group consisting of: 
       
       
         
           
           
               
               
           
         
       
       wherein:
 m is an integer selected from the group consisting of 1, 2, 3, 4, 5, 6, 7 and 8; 
 each R 5  is independently H or —COOR 6  wherein each R 6  is independently H or a C 1 -C 6  alkyl; 
 n is an integer selected from the group consisting of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12; 
 p is an integer selected from the group consisting of 1, 2, 3, 4, 5, 6, 7 and 8; 
 Ch is a chelating moiety that can comprise one or more metals or radiometals; or 
 a pharmaceutically acceptable salt thereof. 
 
     
     
         10 . The method of  claim 9 , wherein the chelating moiety is selected from the group consisting of: 
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         wherein q is an integer selected from the group consisting of 1, 2, 3, 4, 5, 6, 7 and 8. 
       
     
     
         11 . The method of  claim 9 , wherein the compound is selected from the group consisting of: 
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
       
       wherein
 x is selected from the group consisting of 2 and 3; 
 M is a metal or a radiometal; or 
 a pharmaceutically acceptable salt thereof. 
 
     
     
         12 . The method of  claim 9 , wherein the metal is selected from the group consisting of Gd, Lu, Ac, Bi, Pb, Cu, In, Sc and Y. 
     
     
         13 . The method of  claim 9 , wherein imaging comprises magnetic resonance imaging (MRI) and the nonradioactive metal is Gd-157 (stable isotope). 
     
     
         14 . The method of  claim 9 , wherein the method comprises treating one or more prostate-specific membrane antigen (PSMA) tumors or cells and the radiometal is selected from the group consisting of Lu-177, Ac-225, Bi-212, Bi-213, Pb-212, Cu-67, In-111, Sc-47, and Y-90. 
     
     
         15 . The method of  claim 9 , wherein the imaging comprises positron emission tomography (PET) imaging and the radiometal is selected from the group consisting of Y-86 and Sc-44. 
     
     
         16 . The method of  claim 9 , wherein the imaging comprises single-photon emission computed tomography (SPECT) imaging and the radiometal is selected from the group consisting of Lu-177 and In-111. 
     
     
         17 . The method of  claim 9 , wherein the one or more PSMA-expressing tumors or cells is selected from the group consisting of: a prostate tumor or cell, a metastasized prostate tumor or cell, a lung tumor or cell, a renal tumor or cell, a glioblastoma, a pancreatic tumor or cell, a bladder tumor or cell, a sarcoma, a melanoma, a breast tumor or cell, a colon tumor or cell, a germ cell, a pheochromocytoma, an esophageal tumor or cell, a stomach tumor or cell, and combinations thereof. 
     
     
         18 . The method of  claim 9 , wherein the one or more PSMA-expressing tumors or cells is a prostate tumor or cell. 
     
     
         19 . The method of  claim 9 , wherein the one or more PSMA-expressing tumors or cells is in vitro, in vivo, or ex vivo. 
     
     
         20 . The method of  claim 9 , wherein the one or more PSMA-expressing tumors or cells is present in a subject. 
     
     
         21 . The method of  claim 20 , wherein the compound comprising the imaging agent is cleared from the tumor or cell in the subject. 
     
     
         22 . The method of  claim 20 , wherein the compound comprising the imaging agent is selected from the group consisting of: 
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         wherein M is a metal or a radiometal; or 
         a pharmaceutically acceptable salt thereof; 
       
       and is cleared more rapidly from a subject's kidneys than from a tumor of the subject.